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Decoding Cells — The Blog

Scan on Demand

October 10th, 2018 | Debi Chakraborty

Use Scan on Demand to image plates at another objective and linking those scans together using the Add Scans feature in the software. This can provide additional information about morphology and help to easily identify contamination or other plating issues over time without the need to remove the plate from the incubator and look at it under a manual scope.

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Expert view: New developments in real‑time live‑cell analysis

September 5th, 2018

Live-cell analysis is now a well-established method that is part of the everyday cell biologist’s armoury for understanding cell biology. For over a decade, the functionality, throughput, and ease of use offered by real-time live-cell analysis has provided a platform for accurate and reproducible cell biology analysis in multiple biomedical research areas, including oncology, immuno-oncology, immunology and now neuroscience.

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Capture Protein Expression Changes Over Time in Living Cells

August 23rd, 2018

Immunocytochemistry (ICC) as a basic cell imaging technique has been engrained into every cell biologist’s repertoire. Fluorescently labeled antibodies added onto fixed cells help visualize protein expression in the cells. While traditional ICC yields information on protein localization i.e. where the protein of interest is expressed, a big caveat remains – it is unable to capture protein expression changes over time, the ‘when’ of cellular dynamics.

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The Immune System Reacts to Neurological Stress: Catching Killer T Cells Live in Action

April 6th, 2018

Reactive oxygen species (ROS) have long suffered a bad rap. An overdose of ROS is detrimental and causes a state of oxidative stress. But eliminating them isn’t the answer either as our bodies need ROS for basic survival. Moreover, ROS are essential to immune system function, and act to combat perceived threats to the body. However, a lot is unknown about the role of ROS signaling in immune responses, especially in a diseased state.

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Measure Nothing to Measure Something...

December 15th, 2016

…my former boss used to say to me. Not sure whether he was intentionally trying to confuse, but it took a while and several rounds of explanation for me to finally get his point. He referred specifically to work on developing new high-throughput methods for measuring cell behaviour. Not an easy task but something he and colleagues had proven highly successful at.

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Beware the Specification Trap

November 3rd, 2016

Comparing the specifications of things is a powerful approach to help us to decide what to buy. Bigger, smaller, faster, simpler – if you have the spec data at your fingertips decision making is easy, right? Well, only if the data is objective and trustworthy, and only if you know exactly what parameters are important to you and how to weight them. And how do you score more subjective things like usability and aesthetics? Obvious points I know, but there’s more to this than meets the eye…

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