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Video: Utilizing Advanced Flow Cytometry to Detect the Uptake and Transport of Small Molecules by Microbes

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In this video, Dr. Douglas Kell Research Chair in Systems Biology at the University of Liverpool and his team, sat down with us to discuss the role of advanced flow cytometry in their research projects.

Dr. Kell’s research focuses on the transport of small molecules across cellular membranes. The group uses advanced flow cytometry to detect this “uptake” of small fluorescent molecules (fluorophores) into cells.

Dr. Grixti - Research Associate, University of Liverpool describes, her aim is to determine the molecular transporters responsible for uptake of psychoactive drugs into mammalian cells. Interestingly, the group has discovered that fluorophores mimic the chemical structure of many small molecule pharmaceuticals, and Dr. Grixti exploits this characteristic in her research by performing competitive assays between the drugs of interest and their fluorphore mimcs.

Dr. Jindal - Post-doctoral Researcher, University of Liverpool describes a project with the aim of developing a rapid, anti-microbial susceptibility test in samples taken from patients with suspected urinary tract infections (UTIs). Here, Dr Jindal uses the fluorophores to detect proliferation of microbial cells after treatment with various antibiotics.

Dr. Sora - Post-doctoral Researcher, University of Liverpool describes a project wherein he screens a library of up to 50 fluorophores for transport into E. coli cells. He also shares the characteristics in the Intellicyt system he finds particularly useful for this purpose.

Watch the video to discover how Dr. Kell’s research group exploits the power and versatility of advanced flow cytometry, to explore the transport of small molecules across cellular membranes!

Small Molecule Discovery | High Content Suspension Cell Phenotypic Screening

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