Live Cell Insights Publications Newsletter
Read our summaries of new and notable research articles featuring the IncuCyte® Live Cell Analysis System, including hand-picked publications for applications and research areas like immunology, oncology, tumor spheroids, and more!
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Senescence in cancer provides a fail-safe mechanism against oncogenic transformation and often functions in humans to prevent cancer. Because senescent cells are biologically distinct from other cells, they can be targeted by specific ‘senolytic drugs’ for eradication.
In these studies, researchers at the Netherlands Cancer Institute tested the possibility of utilizing a “one-two punch” approach for cancer therapy where they first induce senescence selectively in cancer cells and follow up with treatment with senolytic drugs to target and kill the senescent cancer cells. Their findings show:
Read the full paper in Cell Reports, October 2017
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IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent offers a no wash, mix and read solution to enable quantification of apoptosis over time of cells undergoing caspase-3/7 mediated apoptosis.
Chemotaxis of tumor cells and stromal cells in surrounding microenvironment is an essential component of tumor dissemination during progression and metastasis. Chemokines have well-defined roles in directing cell movements necessary for T-cell immune responses and are crucial for the attraction of monocytes and immature dendritic cells (iDCs) to sites of inflammation, and bringing T cells and APCs together. Overall, the need to quantitatively study cell migration has become increasingly important for advancing drug discovery and screening.
Researchers at Bristol-Myers Squibb sought to leverage the IncuCyte® live-cell imaging system for high-throughput and automated chemotaxis/cell migration assays to validate targets and characterize various compounds and their associated behaviors/properties. The authors found:
Read the full paper in SLAS Discovery, September 2017
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IncuCyte® ClearView Chemotaxis assays
Use the IncuCyte® Live-Cell Analysis Platform to study chemotactic cell migration across 2D substrates and directional cell invasion through 3D biomatrix gels.
Bioinformatic approaches that utilize tumor profiling data sets to subcategorize classification of patients into disease subtypes have revolutionized clinical target discovery in oncology, most notably as an alternative approach for identification of targetable loss-of-function or synthetic lethal lesions that lead to cancer, which are not readily druggable.
In a search for next generation treatment options for ovarian cancers, researchers at the Novartis Institutes for Biomedical Research used The Cancer Genome Atlas (TCGA) data set to identify a recurrent repression of the ubiquitin B (UBB) gene in high-grade serous ovarian cancer (HGSOC) and other gynecological cancers. Using bioinformatic analysis and cell lines in which UBB is strongly suppressed, the researchers demonstrated the therapeutic value of the observed UBB silencing event. Their studies showed:
Read the full paper in The Journal of Clinical Investigation, November 2017
FEATURED PRODUCT
IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent offers a no wash, mix and read solution to enable quantification of apoptosis over time of cells undergoing caspase-3/7 mediated apoptosis.
Drug-resistant cell populations within heterogeneous tumors can serve as the founders of disease relapse and remains a barrier for successful cancer treatment. Epigenetic repression of highly repetitive genome elements contributes to the survival of these drug tolerant subpopulations of cancer cells and has been shown to counterbalance drug-induced expression. This balance can promote genomic stability and selective fitness in drug resistant cancer subpopulations where conditions would normally be lethal.
Therefore, it is increasingly important to disrupt the drug-tolerant state and counteract acquired resistance these subpopulations have to anti-cancer drugs to effectively treat cancers in the clinic. Guler et al. investigated the genomes of a cancer cell subpopulation and its survival to treatment with otherwise lethal drugs, known as drug-tolerant persisters (DTPs). The authors found the following:
Read the full paper in Cancer Cell, August 2017
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IncuCyte® NucLight Red Lentivirus Reagent (EF-1 Alpha Promoter, Puromycin selection)
IncuCyte® NucLight Lentivirus Reagents enable efficient, non-perturbing, nuclear labeling of live mammalian cells and are ideal for generating stable cell populations or clones using puromycin or bleomycin selection.
Head and neck squamous cell carcinoma (HNSCC) is the seventh most common cancer worldwide by incidence, with an estimated 600,000 new cases reported each year worldwide. HNSCC are highly resistant to treatment and have a low survival rate and high rate of tumor recurrence. Epidermal growth factor (EGFR) is overexpressed in more than 90% of HNSCC and is a predictor of poor prognosis. Where EGFR is overexpressed in HNSCC, HER2 and HER3 are also expressed.
Little data exists on a HER2-HER3 blockade and invasion/migration in HNSCC. The aim of this study was to explore the effect of a pan-HER blockade on proliferation, migration and invasion of human HNSCC (and corresponding head and neck cancer stem cells, CSCs population) via combined treatment of cetuximab and pertuzumab, in the presence or absence of photo irradiation. The authors found the following:
Read the full paper in International Journal of Molecular Medicine. February 2017
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IncuCyte ® Woundmaker, Software Module and Cell Migration Kit
An integrated solution for real-time visualization and assessment of cell morphology in scratch wound assay, up to six 96-well plates at once – all inside your incubator.
Thapsigargin (Tg), a sesquiterpene lactone, blocks the sarco/endoplasmic reticulum (ER) Ca2+ATPase (SERCA), disrupting normal Ca2+ homeostasis in cells and eventually leading to apoptosis. Due to this apoptotic effect, analogs of Tg are being utilized as cancer therapeutics, but their cytotoxicity has warranted production of prodrugs that can be activated to target specific cancer types.
To optimize Tg prodrug efficacy, Sehgal et al. sought to understand how Tg and Tg analogs interact with and affect SERCA function to induce cell death in prostate and breast cancer cells. To this end, the researchers used a set of O(8)-sidechain-substituted Tg analogs that can be conjugated and utilized for prodrug synthesis, a Boc-protected derivative of 8ADT (Boc-8ADT) analog with a strongly apoptotic effect similar to that of Tg, and EpoTg, a Tg derivative with a high affinity for SERCA but poor ability to induce apoptosis, and demonstrated:
Read the full paper in The Journal of Biological Chemistry, September 2017
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IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock, providing information-rich analysis that is easy to achieve.
Self-organizing 3D tissue engineering allows researchers to obtain tissue and cells that mimic in vivo development. Utilizing iPSC-derived 3D tissues has broad applicability, including the reproduction and modeling of photoreceptor degeneration.
Ito et al. investigated whether induced photoreceptor cell death in mouse iPSC-derived 3D retinal organoids (3D-retinas) by 4-OHT would induce photoreceptor degeneration in mouse retinal implants. A fluorescent live-cell imaging system allowed for the measurement and quantification of degeneration-related properties. The authors reported the following:
Read the full paper in Stem Cell Research, October 2017
FEATURED PRODUCT
IncuCyte® Tumor Spheroid Assays
An integrated solution to automatically track and quantify tumor spheroid formation, growth and death in real time and in 96- and 384-well formats — inside your tissue culture incubator.