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Chemotatic Migration and Invasion Assays for Live-Cell Analysis


Use the IncuCyte® Live-Cell Analysis Platform to study chemotactic cell migration across 2D substrates and directional cell invasion through 3D biomatrix gels.

  • Visualize chemotaxis in real time with fully automated analysis
  • Use the ClearView chemotaxis plate for highly reproducible 96-well kinetic assays
  • Low cell usage ideal for rare, expensive and primary cell populations
  • Chemotactic gradients are stable and maintained over 72 hours

Chemotactic migration across substrate surfaces

Chemotactic migration across substrate surfaces

  • Monitor and quantify directional cell migration across plastic or biomatrix coatings in real time
  • Validated with a wide range of immune, tumor and vascular cell types
  • Study the effect of treatments on full time course chemotaxis profiles

Learn more

Chemotactic invasion through 3D biomatrix gels

Chemotactic invasion through 3D biomatrix gels

  • Real time imaging and quantification of cell invasion through 3D biomatrix gels
  • Assess metastatic potential and define the effect of treatments on invasive phenotype.
  • Explore differential biology of chemotactic cell migration and invasion in the same plate.

Learn more

Chemotatic transendothelial migration of leukocytes

Chemotatic transendothelial migration of leukocytes

  • Monitor and quantify directional leukocyte migration across an endothelial monolayer in real time
  • Visualize endothelial monolayer integrity and leukocyte diapedesis
  • Investigate the biological effects of inhibitors and neutralizing antibodies

Learn more


3rd Edition! Live-Cell Analysis Handbook

A guide to real-time live-cell imaging & analysis


Reserve your copy

Application Note:

Gain additional insights into the mechanism of immune cell killing by combining live cell analysis and flow cytometry into a single workflow

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Commonly used approaches

In vitro assays for cell migration and chemotaxis are necessary for both basic-research and drug discovery. The table below shows capabilities and challenges of some common approaches.

Existing Boyden Chambers Microfluidic Devices IncuCyte® Chemotaxis Cell Migration and Invasion
Chemotactic migration
Migration in the absence of chemotactic gradients
Low cell usage (<5,000 per well)
Suitable for non-adherent cells
Migration across a surface
Invasion through a 3D gel matrix
Integrated quantitation
Integrated cell observation
Precision, reproducibility
96-well throughput or higher
Kinetic read-out
Label-free
Workflow: set up and walk away
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