Measure Chemotactic Migration, Invasion and Transendothelial Migration in Real Time

Use the IncuCyte® Live-Cell Analysis System to study chemotactic cell migration across 2D substrates, directional cell invasion through 3D biomatrix gels and directional leukocyte migration across the endothelium.

• Visualize chemotaxis in real time with fully automated analysis
• Use the ClearView chemotaxis plate for highly reproducible 96-well kinetic assays
• Low cell usage ideal for rare, expensive and primary cell populations
• Chemotactic gradients are stable and maintained over 72 hours

Commonly used approaches

In vitro assays for cell migration and chemotaxis are necessary for both basic-research and drug discovery. The table below shows capabilities and challenges of some common approaches.

	Existing Boyden Chambers	Microfluidic Devices	IncuCyte® Chemotaxis Cell Migration and Invasion
Chemotactic migration
Migration in the absence of chemotactic gradients
Low cell usage (<5,000 per well)
Suitable for non-adherent cells
Migration across a surface
Invasion through a 3D gel matrix
Integrated quantitation
Integrated cell observation
Precision, reproducibility
96-well throughput or higher
Kinetic read-out
Label-free
Workflow: set up and walk away