Immune Cell Activation Assays

In response to infectious microorganisms such as bacteria, viruses and fungi activated cells of the immune system undergo rapid expansion and participate in cell-cell interactions to help regulate the effect and extent of the immune response. The IncuCyte live-cell analysis system enables real-time monitoring of immune cell proliferation and cell health, analysis of dynamic immune cell–cell interactions and the study of immune signaling pathways.

IncuCyte® Immune cell clustering & proliferation assays

Visualize and quantify immune cell interactions and proliferation in real time
Investigate the mechanisms of immune cell activation (proliferation), regulation and differentiation (clustering) without the need to lift or label your cells

Learn more about immune cell clustering & proliferation assays

Activation of PBMCs with anti-CD3, anti-CD28 and IL-2 induces T cell aggregation or "clustering". Compare different combinations of activation stimuli (e.g. CD3, CD28, IL-2) and reveal how the onset time, rate and extent of proliferation and clustering are dependent on activators and concentrations used.

Continuous analysis of immune cell health

IncuCyte® mix-and-read reagents enable multiplexed analysis of cytotoxicity and apoptosis in immune cell cultures

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Jurkat T lymphocytes treated with the cytotoxic agent camptothecin in the presence of IncuCyte® Annexin V Red reagent which labels apoptotic cells in real-time

Jurkat T lymphocytes treated with the cytotoxic agent camptothecin in the presence of IncuCyte® Annexin V Red reagent which labels apoptotic cells in real-time. Concentration response curves were generated from IncuCyte® live cell analysis time-course profiles to enable determination of IC₅₀ values.

IncuCyte® Reporter assays - investigate immune response signalling pathways

Measure signalling pathway activation in real-time via transcriptional fluorescent reporter genes
Assess transcription factors and associated signalling pathways that are regulated by specific genes or drugs

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Evaluate the entire time course of activation in one experiment

Evaluate the entire time course of activation in one experiment. Time-course of NF-κB driven rhGFP reporter gene expression following rhTNF-α stimulation in HEK 293 cells.