In response to infectious microorganisms such as bacteria, viruses and fungi activated cells of the immune system undergo rapid expansion and participate in cell-cell interactions to help regulate the effect and extent of the immune response. The IncuCyte live-cell analysis system enables real-time monitoring of immune cell proliferation and cell health, analysis of dynamic immune cell–cell interactions and the study of immune signaling pathways.
Activation of PBMCs with anti-CD3, anti-CD28 and IL-2 induces T cell aggregation or "clustering". Compare different combinations of activation stimuli (e.g. CD3, CD28, IL-2) and reveal how the onset time, rate and extent of proliferation and clustering are dependent on activators and concentrations used.
Jurkat T lymphocytes treated with the cytotoxic agent camptothecin in the presence of IncuCyte® Annexin V Red reagent which labels apoptotic cells in real-time. Concentration response curves were generated from IncuCyte® live cell analysis time-course profiles to enable determination of IC50 values.
Evaluate the entire time course of activation in one experiment. Time-course of NF-κB driven rhGFP reporter gene expression following rhTNF-α stimulation in HEK 293 cells.