Immune Cell Activation Assays

Immune Cell Activation Assays

In response to infectious microorganisms such as bacteria, viruses and fungi activated cells of the immune system undergo rapid expansion and participate in cell-cell interactions to help regulate the effect and extent of the immune response. The IncuCyte live-cell analysis system enables real-time monitoring of immune cell proliferation and cell health, analysis of dynamic immune cell–cell interactions and the study of immune signaling pathways.

Contact us for more information    Learn more about IncuCyte Immune Cell Assays


IncuCyte® Immune cell clustering & proliferation assays

  • Visualize and quantify immune cell interactions and proliferation in real time
  • Investigate the mechanisms of immune cell activation (proliferation), regulation and differentiation (clustering) without the need to lift or label your cells

Learn more about immune cell clustering & proliferation assays

Immune Cell Clustering Video

Activation of PBMCs with anti-CD3, anti-CD28 and IL-2 induces T cell aggregation or clustering

Activation of PBMCs with anti-CD3, anti-CD28 and IL-2 induces T cell aggregation or "clustering". Compare different combinations of activation stimuli (e.g. CD3, CD28, IL-2) and reveal how the onset time, rate and extent of proliferation and clustering are dependent on activators and concentrations used.

Continuous analysis of immune cell health

  • IncuCyte® mix-and-read reagents enable multiplexed analysis of cytotoxicity and apoptosis in immune cell cultures

Learn more about cell health assays

Jurkat T lymphocytes treated with the cytotoxic agent camptothecin in the presence of IncuCyte® Annexin V Red reagent which labels apoptotic cells in real-time

Jurkat T lymphocytes treated with the cytotoxic agent camptothecin in the presence of IncuCyte® Annexin V Red reagent which labels apoptotic cells in real-time. Concentration response curves were generated from IncuCyte® live cell analysis time-course profiles to enable determination of IC50 values.

IncuCyte® Reporter assays - investigate immune response signalling pathways

  • Measure signalling pathway activation in real-time via transcriptional fluorescent reporter genes
  • Assess transcription factors and associated signalling pathways that are regulated by specific genes or drugs

Learn more about reporter assays

Evaluate the entire time course of activation in one experiment

Evaluate the entire time course of activation in one experiment. Time-course of NF-κB driven rhGFP reporter gene expression following rhTNF-α stimulation in HEK 293 cells.

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