Applications

Reporter Genes

Use IncuCyte® Live-Cell Analysis System to measure signalling pathway activation via transcriptional fluorescent reporter genes. Measure activity of any promoter driven recombinant GFP/RFP reporter gene expression in real time.


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Key Features

Key Features

Fully kinetic measurements

Measure temporal expression of GFP/RFP reporter gene following target or pathway stimulation (e.g. NF-kappaB). Real-time kinetic data offers insights not attainable with end-point assays

Straightforward & Cost effective

Unlike luciferase assays, does not require a terminal reaction substrate saving time and money. No requirement to define end points a priori

Amenable to 96 and 384-well formats

Provide 576 or 2,304 wells of data per IncuCyte® system at each time point

Validate data with images & movies

Interrogate cell morphology changes by visualizing images and time-lapse movies

Automated image acquisition and processing

Acquire and analyse images using fully integrated software algorithms. Visualize kinetic plots in microplate views. Export data to calculate EC50/IC50 values

Example Data

Example Data

Left: IncuCyte fluorescent image of HEK293 cells transiently transfected with a commercially available reporter gene construct (pNF-kB-rhGFP). Representative fluorescent image taken at 20h of cells treated with rhTNF-alpha (11 ng/ml). Right: Time-course of induction of NF-kB driven rhGFP reporter gene expression following rhTNF-alpha stimulation in HEK293 cells (n=5 wells).

IncuCyte fluorescent image of HEK293 cells transiently transfected with a commercially available reporter gene construct

Time-course of induction of NF-kB driven rhGFP reporter gene expression following rhTNF-alpha stimulation in HEK293 cells

Technical Resources

Technical Resources

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