An Optimized Multiplex Assay for Screening the Functional Capacity of Bispecific T Cell Engager (BiTE) Induced Killing, using Advanced Flow Cytometry
Cytotoxic T cell lymphocytes become activated upon recognition of cancer or damaged cells, and release effector proteins to initiate the killing of target cells.
Monitoring this process may help in assessing the efficacy of small molecules and biologics, intended for use within the cell therapeutics field.
This poster video describes a simple T cell mediated killing assay workflow, which uses the iQue® Advanced Flow Cytometry Platform, Human T Cell Killing Kit and iQue Forecyt® software.
Watch the narration of this video poster:
Further Resources:
- iQue® Human T Cell Killing Kit
- Forecyt® software – interactive assay development, analysis and multiparameter data visualizatio
- Compendium of Posters: iQue® Advanced Flow Cytometry Platform Application Posters: Antibody Discovery and Immuno-Oncology
- Application Note: Combining Live-Cell Analysis and High-Throughput Flow Cytometry to Gain Additional Insights into the Mechanisms of Immune Cell Killing of Tumor Cells