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Software: Identify where the cells within your gate fall in the all events plot, using the "Back Gate"
December 13th, 2019
Back gating helps to identify where the cells within your gate fall, within the all events plot. Back gating helps confirm your gating strategy, particularly for forward scatter vs. side scatter.
View articleUncovering the Secrets of Cellular Motility: The Impact of Actin Nt-Acetylation on the Cytoskeleton
December 9th, 2019
Cellular motility in animals is a dynamic process, dependent on the interactions of microfilaments (e.g. actin and myosin), intermediate filaments (e.g. keratin), and microtubules. Working together in concert, these cytoskeletal structures engage in cellular activities such as cellular migration, transportation of vesicles, and gene transcription to name a few. In particular, the actin protein is a busy player; shuffling states between polymerization at the leading edge of cells to help pull them forward, and depolymerization at the trailing edge, pushing them from behind.
View articleLive Cell Analysis for Neuroscience Research
December 5th, 2019
Susana Alcantara, Senior R&D Scientist and Neuroscience Program Leader, BioAnalytics, of Sartorius, chatted with Brandy Sargent of Cell Culture Dish about how real-time live cell analysis is enabling neuroscience research.
View articleCRISPR: uncovering clues with off-target therapeutic implications
November 7th, 2019
Exploiting bacterial defense mechanisms has led to the development of CRISPR technology for gene editing and identification. CRISPR has become a popular research tool in the fight against cancer, neurological disease, heart failure, and other rare conditions in desperate need of new therapeutic options. With new technology, however, comes new questions: one being how to best to monitor possible off-target effects that might arise from experimental manipulations and determine the factors that contribute? This is not an easy question to answer.
View articleSoftware: Automatically identify populations of interest with 'Smart Gate'
October 29th, 2019
Gates used during flow cytometry data analysis aren’t 100% accurate even with a template. The integrated ForeCyt® Software on the iQue3 advanced flow cytometry platform addresses this issue with “Smart gate”, which automatically identifies the population of interest by centering itself on the closest dense area on the graph.
View articleReal-time visualization and quantification of neutrophil activation and function using Live-Cell Analysis
October 18th, 2019
Here’s a chance to see a poster presentation come to life. The poster was originally displayed at the annual meeting of the American Association of Immunologists (AAI) 2019.
View articleThe Trouble with ELISA
September 25th, 2019
In this article, we discuss some of the current challenges of the ELISA assay, some alternatives for immunoassay (particularly flow cytometry), and some tools and technologies that can address the limitations of ELISA and other immunoassays.
View articleHow Scientists are Working to Fight the Deadliest Form of Cancer with Oncolytic Viruses
September 12th, 2019
Dr. Pierre Cordelier describes how some exciting pre-clinical data suggests oncolytic viruses could tackle pancreatic cancer
View articleAntibody Screening Tools, Improving Workflows
August 14th, 2019
Helping researchers identify, characterize and investigate new antibody candidates, fuels the growing demand for therapeutic antibodies.
View articleImmune Cell Killing, Multi-Spheroids
August 6th, 2019
It is captivating to observe how Herceptin alone, or peripheral blood mononuclear cells (PBMCs) alone, have little or no effect on multi-spheroid size or viability (fluorescence intensity) of SKOV-3 multi-spheroid proliferations.
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