Webinar Recording:

Longitudinal Characterization of Human Stem Cell-Derived Neurons Using Calcium Imaging

The ability to measure neuronal activity and network activity in a longitudinal manner is paramount to the study of neurological disease Due to the synaptic plasticity of neuronal cell cultures, identifying dynamic changes between normal and mutated models is critical for understanding changes in network connectivity. To evaluate activity differences between normal and diseased states, we used mutant and control human induced pluripotent stem cells (iPSCs) to generate neuronal cultures by forcing expression of Neurogenin-2. Using the IncuCyte® S3 live-cell analysis system, we measured neuronal activity over the course of several weeks for functional analysis of differences in activity and connectivity. We found that iPSC-derived neurons had increased activity over time and formed synchronous neuronal networks around 3 weeks of differentiation. Data generated showed a reproducible, relevant phenotypic readout for long-term network changes in this epileptic cell model.

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Louis Dang

Presenter: Louis Dang, MD/PhD

Clinical Lecturer, University of Michigan

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