IncuCyte® CytoLight Reagents enable efficient, non-perturbing, cytoplasmic labeling of living cells. They are a unique range of lentiviral-based labeling reagents with simple transduction protocols that enable expression of a cytoplasmically soluble green (GFP) or red (mKate2) fluorescent protein in your choice of primary, immortalized, dividing, or non-dividing cells.
These labeling reagents have been validated for use with the IncuCyte® Live-Cell Analysis System and are ideal for monitoring and quantifying the real-time formation of multicellular structures and cell-cell interactions in co-culture systems.
Label cells without altering cell function and monitor live-cell interactions over days and weeks. 3D-spheroid formation and growth using A549 tumor cells labeled with IncuCyte® CytoLight Red Reagent (left) and vascular tube formation in a co-culture model of angiogenesis using HUVEC primary endothelial cells labeled with IncuCyte® CytoLight Green Reagent (right). Note that cells remain healthy and fluorescence expression is maintained over the entire time course (>10 days).
Researchers around the world are using IncuCyte® CytoLight Reagents to monitor and quantify the formation of multicellular structures in complex culture models. Explore the IncuCyte® publications library and learn why IncuCyte® technology has been sited in over 700 peer reviewed publications.
|Name||Type||Promoter||Selection||Ex. maxima||Em. maxima||Catalog No.|
|IncuCyte® CytoLight Green||Lentivirus||EF-1α||Puromycin||483 nM||506 nM||4481|
|IncuCyte® CytoLight Red||Lentivirus||EF-1α||Puromycin||588 nM||633 nM||4482|
|IncuCyte® CytoLight Green||Lentivirus||CMV||None||483 nM||506 nM||4513|