IncuCyte® CytoLight Reagents


IncuCyte® CytoLight Reagents enable efficient, non-perturbing, cytoplasmic labeling of living cells. They are a unique range of lentiviral-based labeling reagents with simple transduction protocols that enable expression of a cytoplasmically soluble green (GFP) or red (mKate2) fluorescent protein in your choice of primary, immortalized, dividing, or non-dividing cells.

These labeling reagents have been validated for use with the IncuCyte® Live-Cell Analysis System and are ideal for monitoring and quantifying the real-time formation of multicellular structures and cell-cell interactions in co-culture systems.

3D-Spheroid Formation, Growth and Shrinkage Using the IncuCyte® Live-Cell Analysis System

3D-Spheroid Formation, Growth and Shrinkage Using the IncuCyte® Live-Cell Analysis System

Real-Time Monitoring of Angiogenesis Using the IncuCyte® Live-Cell Analysis System

Real-Time Monitoring of Angiogenesis Using the IncuCyte® Live-Cell Analysis System

Label cells without altering cell function and monitor live-cell interactions over days and weeks. 3D-spheroid formation and growth using A549 tumor cells labeled with IncuCyte® CytoLight Red Reagent (left) and vascular tube formation in a co-culture model of angiogenesis using HUVEC primary endothelial cells labeled with IncuCyte® CytoLight Green Reagent (right). Note that cells remain healthy and fluorescence expression is maintained over the entire time course (>10 days).


IncuCyte® CytoLight Reagents – IncuCyte® Publications

Researchers around the world are using IncuCyte® CytoLight Reagents to monitor and quantify the formation of multicellular structures in complex culture models. Explore the IncuCyte® publications library and learn why IncuCyte® technology has been sited in over 700 peer reviewed publications.

Phosphoproteomic analysis implicates the mTORC2-FoxO1 axis in VEGF signaling and feedback activation of receptor tyrosine kinases.
Zhuang G, Yu K, Jiang Z, Chung A, Yao J, Ha C, Toy K, Soriano R, Haley B, Blackwood E, Sampath D, Bais C, Lill JR, Ferrara N.
Science Signaling
6 (271):ra25 (2013);

Researchers at Genentech used HUVEC cells transduced with the IncuCyte® CytoLight™ Green reagent in a co-culture model of angiogenesis to reveal a link between FOXO1 inhibition of VEGF signaling and the PI3K-mTORC2 signally axis.


Label and monitor cell-cell interactions without perturbing function or morphology

  • Monitor the assembly of multicellular structures in real time over days or weeks
  • Non-perturbing to cell biology and function
  • Simple labeling protocols – compatible with your choice of cells
  • Validated for use with the IncuCyte® Live-Cell Analysis System
  • Ideal for co-cultures and multiplexing with IncuCyte® apoptosis or cytotoxicity assays
IncuCyte® CytoLight Lentivirus Reagents

IncuCyte® CytoLight Lentivirus Reagents

  • Long term, homogeneous expression
  • Simple ‘seed-transduce-select’ protocols
  • Ideal for generating stable cell populations

IncuCyte® CytoLight Lentivirus Reagents

Name Type Promoter Selection Ex. maxima Em. maxima Catalog No.
IncuCyte® CytoLight Green Lentivirus EF-1α Puromycin 483 nM 506 nM 4481
IncuCyte® CytoLight Red Lentivirus EF-1α Puromycin 588 nM 633 nM 4482
IncuCyte® CytoLight Green Lentivirus CMV None 483 nM 506 nM 4513

IncuCyte® CytoLight Pre-labeled Cell Lines

Direct access to cells stably expressing NucLight™ Green or Red fluorescent proteins. No need to transduce. Simply thaw and use.

Name Selection Volume Ex. maxima Em. maxima Catalog No.
HUVEC CytoLight Green Cells None 1.7x105 cells/vial 483 nM 506 nM 4453

Related Applications

3D-Spheroid

3D-Spheroids

Use IncuCyte ZOOM® to track and quantify non-adherent spheroid formation, growth and shrinkage.

Learn more

Angiogenesis

Angiogenesis

Kinetically measure vascular tube formation using our fully integrated solution and advanced cell models

Learn more

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