Viral replication and infection assays enable the study of pathogenesis in host cultures and the testing of novel antiviral therapeutics. Using simple and reliable quantification, IncuCyte® live-cell analysis enables real-time analysis of infection and replication providing a platform for drug discovery.
Time-course of nuclear GPF expression in A549 human lung carcinoma cells transduced with IncuCyte® NucLight Green lenti- virus. Transduction of cells with NucLight Green is titratable (HUVECs).
BHK-21 cells were cotransfected with two foot-and-mouth disease virus (FMDV) replicons containing either a GFP (green) or mCherry (red) reporters. IncuCyte® coincident object analysis was used to quantify the proportion of cells expressing one (green or red) or both (yellow) of the replicons over time. Data courtesy of Dr. Morgan Herod (University of Leeds).
In this webinar you will learn: