Viral Replication and Infection Assays

Viral Replication and Infection Assays

Viral replication and infection assays enable the study of pathogenesis in host cultures and the testing of novel antiviral therapeutics. Using simple and reliable quantification, IncuCyte® live-cell analysis enables real-time analysis of infection and replication providing a platform for drug discovery.

Contact us for more information    Learn more about IncuCyte Immune Cell Assays


Real time analysis of viral infection

  • Fluorescent ‘reporter’ proteins enable quantification of virus infection
  • Compare infection time-courses and efficiencies

Time-course of nuclear GPF expression in A549 human lung carcinoma cells transduced with IncuCyte® NucLight Green lenti- virus

Time-course of nuclear GPF expression in A549 human lung carcinoma cells transduced with IncuCyte® NucLight Green lenti- virus. Transduction of cells with NucLight Green is titratable (HUVECs). 

Investigate infection modulation and synergy 

  • Study factors that promote coinfection

BHK-21 cells were cotransfected with two foot-and-mouth disease virus (FMDV) replicons containing either a GFP (green) or mCherry (red) reporters

BHK-21 cells were cotransfected with two foot-and-mouth disease virus (FMDV) replicons containing either a GFP (green) or mCherry (red) reporters. IncuCyte® coincident object analysis was used to quantify the proportion of cells expressing one (green or red) or both (yellow) of the replicons over time. Data courtesy of Dr. Morgan Herod (University of Leeds).


fiona Webinar: Furthering Insight and Productivity in Cell Biology with Real-Time Quantitative Live-Cell Analysis

Webinar: Furthering Insight and Productivity in Cell Biology with Real-Time Quantitative Live-Cell Analysis

In this webinar you will learn:

  • How live cell analysis enables the study of FMDV RNA replication without the need for high level bio-containment facilities.
  • How fluorescent ‘reporter’ proteins allow us to monitor RNA replication by live-cell imaging / image analyses.
  • How you can use live-cell analysis and imaging, inside your incubator, to handle sensitive experiments

Register and watch now

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