Immune-cell recognition, killing and clearance of diseased cells and pathogens are critical components of the human host defense system. The IncuCyte live-cell analysis system enables real-time visualization and measurements of T cell killing and the phagocytic clearance of pathogens or dying cells by phagocytes.
Real-time monitoring of immune cell killing. IncuCyte movies showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. Labeling of the target cells with IncuCyte® NucLight Red Live-Cell Labeling reagent enables direct counting of viable target cell numbers over-time. Addition of the IncuCyte® Caspase 3/7 apoptosis reagent enables simultaneous detection of cells undergoing apoptosis (green fluorescence).
Real-time monitoring of phagocytosis. IncuCyte® pHrodo® Green E. coli Bioparticles® engulfed by the mouse macrophage cell type J774A.1. The Bioparticles® are phagocytosed and, on entering the acidic environment of the phagosome, increase in fluorescence. Note the dynamic activity of the macrophages and engulfment of bioparticles over time via the formation of phagocytic cups.