AAI 2017 - Immunology 2017
May 12 – 16, 2017
Washington D.C. - Walter E. Washington Convention Center
Essen Booth #442
Visit our booth at AAI to learn NEW Next-Generation IncuCyte® S3 Live-Cell Analysis System and the latest trends and applications in live-cell analysis.
Change can happen in an instant. Find out how the IncuCyte® S3 System and IncuCyte® reagents and consumables make it easier than ever to visualize cell behavior and quantify cell function in real time. Derive deeper and more physiologically relevant information about your cells, plus real-time kinetic data, without ever removing your cells from the incubator.
Also, stop by for a chance to win prizes! Spin the wheel of fortune or test your cell culture genius with our raffle quiz to win t-shirts, mugs, and more.
At the show, visit our poster presentation, led by Dr. Dan Appledorn, Director of Biology, showing innovative new research from Essen BioScience highlighting the powerful insights gained from IncuCyte® live-cell analysis. Or join our exhibitor workshop, also presented by Dr. Appledorn, to learn the benefits of incorporating real-time, automated cell imaging and analysis into your in vitro assay workflow for immunology research.
81.15 CD47 antibody-induced engulfment of human T cell leukemia cells by bone marrow-derived macrophages
Presenter: Dr. Daniel Appledorn, Director of Biology
Session Date and Time: Saturday May 13, 2017 2:30 PM - 3:45 PM
Location: Exhibit/Poster Hall, Session: Technological Innovations I
Authors: Daniel Appledorn1, Gillian Lovell1, Clare Szybut1, Kalpana Patel1, Hinnah Campwala1, Nicola Bevan1, Tim Dale1, Derek Trezise1. 1Essen BioScience
CD47 is a trans-membrane “don’t-eat-me” signaling protein that enables tumor cells to evade clearance by neighboring phagocytes. Blocking CD47 allows phagocytes to clear tumor cells and is a promising new approach for cancer immunotherapy. In this study, we characterized anti-CD47 antibody-mediated engulfment of living tumor cells (CCRF-CEM) by mouse bone-marrow derived macrophages (BMDMs) or immortalized mouse macrophages (J774A.1). Phagocytosis was quantified using a pH-sensitive cell-labeling fluorescent probe, pHrodo, and automated kinetic live-cell analysis (IncuCyte). CCRF-CEM cells were labeled, washed and then treated with antibody. Target cells were then added to BMDMs or J774A.1 cells in 96-well plates. Phase- and fluorescence images were captured and quantified over time. Anti-CD47 antibody, but not IgG-control, produced time- and concentration-dependent engulfment of CCRF-CEMs by BMDMs (30’-4h), as evidenced by a significant increase in intracellular fluorescence. Close inspection of the time-lapse images revealed clear cellular engulfment coincident with the appearance of the fluorescent signal. Similar observations were made with J774A.1 macrophages. Interestingly, the rate and degree of engulfment was effector cell-dependent. The mechanism of engulfment was not via induction of target cell apoptosis since anti-CD47 did not induce PS externalization (Annexin V) or activate caspase 3/7. Our experimental findings substantiate the known pro-phagocytic effects of anti-CD47 antibodies, and provide a model system and method for quantitative functional analysis and mechanistic insight of CD47 modulators as cancer therapeutics.
Live Cell Assay Approaches for Immunology and Immuno-oncology: Cell Health, Chemotaxis, Immune Cell Killing, and More
Monday, May 15, 2017
12:30 PM – 1:15 PM
EXHIBITOR WORKSHOP ROOM 2
Presenter: Dan Appledorn, Ph.D., Director of Biology, Essen Bioscience
Join this workshop to learn how to add real-time visualization and analysis of immune cell cultures to your in vitro assay toolkit for immunology research. The IncuCyte® Live-Cell Analysis System continuously analyzes cell activity within your incubator, providing insights into the complex and dynamic cellular processes of immunology. Unlike traditional approaches, which are mostly endpoint, invasive, and labor intensive, the IncuCyte reagents, protocols, and high-throughput-enabled assays are kinetic, simple, and cell sparing—ready to complement your current assay and cell biology workflows. This workshop will showcase real-time, live-cell assays for immune cell activation, proliferation, chemotaxis, transendothelial migration, immune cell killing, and phagocytosis.