Reporter Genes
Use IncuCyte® Live-Cell Analysis System to measure signaling pathway activation via transcriptional fluorescent reporter genes. Measure activity of any promoter driven recombinant GFP/RFP reporter gene expression in real time.
Key Advantages
Key Advantages
Fully kinetic measurements
Measure temporal expression of GFP/RFP reporter gene following target or pathway stimulation (e.g. NF-kappaB). Real-time kinetic data offers insights not attainable with end-point assays
Straightforward & Cost effective
Unlike luciferase assays, does not require a terminal reaction substrate saving time and money. No requirement to define end points a priori
Amenable to 96 and 384-well formats
Provide 576 or 2,304 wells of data per IncuCyte® system at each time point
Validate data with images & movies
Interrogate cell morphology changes by visualizing images and time-lapse movies
Automated image acquisition and processing
Acquire and analyze images using fully integrated software algorithms. Visualize kinetic plots in microplate views. Export data to calculate EC50/IC50 values
Example Data
Left: IncuCyte fluorescent image of HEK293 cells transiently transfected with a commercially available reporter gene construct (pNF-kB-rhGFP). Representative fluorescent image taken at 20h of cells treated with rhTNF-alpha (11 ng/ml).
Right: Time-course of induction of NF-kB driven rhGFP reporter gene expression following rhTNF-alpha stimulation in HEK293 cells (n=5 wells).
