Video: IncuCyte Poster Snapshots | Quantitative Live-Cell Imaging Assays for Immunotherapy
Poster: Quantitative live-cell imaging assays for immunotherapy: chemotaxis, immune cell killing & phagocytosis
G. Lovell, N. Bevan, C. Szybut, N. Rodinova, D. Appledorn, M. Tikhonenko, L. O’Clair, T. O’Callaghan, T. Dale & D. Trezise. Essen BioScience R&D, Welwyn Garden City, UK & Ann Arbor, Michigan, USA
Presented at CIMT 2016, Germany
Summary & impact:
- For the body to defend and fight against cancer, immune cells must recognise, engage, destroy and ultimately remove unwanted tumour cells. Understanding
- these processes and interactions at the cellular level is central to identifying and validating new drug targets and cellular therapy approaches.
- Essen BioScience offers a flexible range of phenotypic assays to explore all aspects of the immuno-oncology research area. All of these assays are based on noninvasive live-cell analysis of cells in 96-well micro-plates using an IncuCyte® ZOOM live cell analysis system.
- Here we describe a cluster of new assays for quantifying immune cell biology and interactions with tumour cells.
- Phase-contrast/fluorescence images from cells gathered over time are processed to measure phenotypes, such as immune cell proliferation, migration & death.
- Experiments are performed with low cell numbers using simple mix and read formats which are non-perturbing to the cell model.
- Each of these approaches provide a full time-course of the biology; images and time-lapse movies provide credibility and valuable biological insight. Added throughput and automated image analysis enhances productivity.