IncuCyte® CytoLight Reagents

IncuCyte® CytoLight Reagents enable efficient, non-perturbing, cytoplasmic labeling of living cells. They are a unique range of lentiviral-based labeling reagents with simple transduction protocols that enable expression of a cytoplasmically soluble green (GFP) or red (mKate2) fluorescent protein in your choice of primary, immortalized, dividing, or non-dividing cells.

These labeling reagents have been validated for use with the IncuCyte® Live-Cell Analysis System and are ideal for monitoring and quantifying the real-time formation of multicellular structures and cell-cell interactions in co-culture systems.

Label cells without altering cell function and monitor live-cell interactions over days and weeks. 3D-spheroid formation and growth using A549 tumor cells labeled with IncuCyte® CytoLight Red Reagent (left) and vascular tube formation in a co-culture model of angiogenesis using HUVEC primary endothelial cells labeled with IncuCyte® CytoLight Green Reagent (right). Note that cells remain healthy and fluorescence expression is maintained over the entire time course (>10 days).

IncuCyte® CytoLight Reagents – IncuCyte® Publications

Researchers around the world are using IncuCyte® CytoLight Reagents to monitor and quantify the formation of multicellular structures in complex culture models. Explore the IncuCyte® publications library and learn why IncuCyte® technology has been sited in over 700 peer reviewed publications.

Label and monitor cell-cell interactions without perturbing function or morphology

• Monitor the assembly of multicellular structures in real time over days or weeks
• Non-perturbing to cell biology and function
• Simple labeling protocols – compatible with your choice of cells
• Validated for use with the IncuCyte® Live-Cell Analysis System
• Ideal for co-cultures and multiplexing with IncuCyte® apoptosis or cytotoxicity assays

IncuCyte® CytoLight Lentivirus Reagents

• Long term, homogeneous expression
• Simple ‘seed-transduce-select’ protocols
• Ideal for generating stable cell populations

IncuCyte® CytoLight Lentivirus Reagents

IncuCyte® CytoLight Pre-labeled Cell Lines

Direct access to cells stably expressing NucLight™ Green or Red fluorescent proteins. No need to transduce. Simply thaw and use.