Establishing the characteristics of cell models is a key component of assay optimization processes performed at AstraZeneca. Learn how to use longitudinal live-cell imaging assays to characterize cells for assay development and assay endpoints. Examples will include how to measure cell confluence and assess the proliferation rate of different cell types to establish optimal assay conditions to screen for cytostatic or cytotoxic compounds in cancer cell lines.
In addition to analysis of cell phenotype using imaging platforms, high-throughput flow cytometry is also being used to gain insights into cell behavior, especially in the immuno-oncology field. Harnessing the immune system to combat cancer is a key interest area for AstraZeneca, and characterizing treatment effects upon immune cell phenotype is a key piece of data in drug discovery workflows. Learn how high-throughput flow cytometry is being used to enable rapid analysis of cells and beads to measure cytokine levels, cell proliferation and cytolytic activity of primary human immune cells.