Optimization of culture conditions and evaluation of cell health
A major limitation in studying human diseases affecting the nervous system is the ability to culture, monitor and analyze neuronal cells that accurately represent human phenotypes of these disorders. The use of human induced pluripotent stem cell (hiPSC)-derived neurons has provided a new approach aimed at modeling neurological diseases. Monitoring neuronal morphology and cell health in long-term culture is critical for the characterization and evaluation of these novel model systems. Traditional approaches rely on endpoint assays and imaging techniques that require immunochemical staining, which provide limited real-time kinetic information. In this webinar, we highlight optimal culture conditions and demonstrate the ability of the IncuCyte S3® approach for real-time, long-term quantitative analysis of iPSC-derived neuronal cell health.
Watch this webinar to learn how:
- You can easily optimize culture conditions for (hiPSC)-derived neurons
- Real-time live-cell analysis using phase (monoculture) or fluorescent (co-culture) NeuroTrack software can be used to visualize and quantify neurite dynamics
- Other live-cell phenotypic assays (e.g. apoptosis) are used to monitor neuronal cell health and validate mechanisms of action
About the speaker
Aaron Overland
Senior Application Scientist
Essen BioScience
Aaron joined Essen BioScience as a Senior Application Scientist in September of 2016 and is leading projects focused on developing new reagents and applications for live cell analysis of neuronal health and function using the IncuCyte system. Aaron earned his BS and PhD in Neuroscience from the University of Minnesota, where he investigated cellular signaling mechanisms mediating analgesic synergy between agonists acting at delta-opioid and alpha2-adrenergic receptors in the spinal cord. His postdoctoral work at University of California, San Diego expanded upon his foundation in cell-based assays of G protein-coupled receptor signaling, focusing on the discovery of a novel signaling module for G protein-mediated transactivation of EFGR in cardiomyocytes and fibroblasts.
