Immuno-oncology (IO) has transformed cancer treatment. The number of treatments in the IO pipeline continues to increase. If the treatment is CAR-T cells, adoptive T cell therapy or checkpoint inhibitors, the mechanism of action includes T cell lysis of tumor cells.
Using fluorescent labels of cytotoxicity, Incucyte® Live-Cell Analysis offers remote monitoring of tumor cell lysis. This webinar presents data using tumor antigen specific T cells from Cellero and commonly available tumor cell lines, to demonstrate specific lysis of tumor cells.
Key webinar discussion topics include:
Information on crucial technical factors
Comparison of Annexin V and Caspase 3/7 for readout and analysis options
The use of adherent vs suspension cells as targets
Controls, and establishing specificity of killing
The importance of plating density of target cells
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Dr. Anne Lodge
Chief Science & Innovation Officer
Anne Lodge received her Ph.D. in Cell and Molecular Biology from the University of Vermont. Her thesis work examined the activation of CD4+ and CD8+ T cells by vascular endothelium and revealed the importance of endothelial cells in triggering organ rejection.
After completing her graduate work, Dr. Lodge received a postdoctoral fellowship from the Multiple Sclerosis Society. The fellowship supported her work at Vanderbilt University in the laboratory of Dr. Subramaniam Sriram. She studied T cells from patients with MS to determine their role in the disease process.
Dr. Lodge then accepted a position with Northwest Biotherapeutics, Inc. in Seattle. She had a number of different roles at the company and contributed to the Investigational New Drug filings for the company’s dendritic cell vaccine. Her expertise in cell-based therapeutics and broad background in immunology led to her founding Astarte Biologics in 2004 to provide immune cell products for research.