How do your immunotherapeutic cells kill a tumour? Laboratory assessment of T cell killing efficiency is debatable: either too artificial or too murine systems. Although not perfect, live cell imaging possesses the advantage to record the event live in a quantifiable manner. Our lab explores several strategies to expand the possibilities to target different types of solid cancer by T cell transfer, including by T cell receptor (TCR) and chimeric antigen receptor (CAR) therapies. However, direct assessment in 2D cell cultures might not represent the optimal validation method.
In addition, in vivo experiments in murine models are fastidious and not always conclusive to determine the efficacy of a human T-cell based treatment. We will present the use of tumour spheres as target for the different type of redirected effector cells (CAR, TCR, NK) and explain how convenient this method is to obtain an important amount of information in a short time.
Dr. Else Marit Inderberg, Group Leader, Oslo University Hospital
Dr. Sébastien Wälchli, Group Leader, Oslo University Hospital