Kinetic antibody internalization assays using live-cell analysis
Monoclonal antibodies (mAb) and antibody-drug conjugates (ADCs) are widely used biological therapeutics. A key property of these biologics is the extent and rate of internalization into different cells, which governs their efficacy, safety and pharmaco-dynamic profile. As a result, quantifying and comparing the rate of Ab internalization of drug candidates is critical in the biopharmaceutical selection and optimization process.
In this mini-webinar Nicola Bevan will discuss:
- How real-time live-cell analysis of antibody internalization can provide additional insight.
- The use of the IncuCyte FabFluor-pH reagent to provide a turn-key approach to antibody labelling.
- Examples of how the assay may be used for characterisation of early stage test antibodies and candidates in a biologics discovery paradigm.
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