In the search for greater clinical translation, cancer researchers are increasingly turning to more complex live-cell assay systems that recapitulate key aspects of the tumor microenvironment. These include (1) more relevant tissue organization, (2) bio-matrices containing structural proteins and growth factors, and (3) the presence of stromal and immune cells. In this webinar, we describe integrated protocols and live-cell analysis solutions for different 3D tumor models, ranging from simple mono-culture single spheroid assays through to multi-spheroid tumor/fibroblast and tumor/immune cell co-cultures in a Matrigel biomatrix.
Kalpana is currently a Principal Scientists within our UK Biology R&D team based at Essen BioSciences European laboratories. A highly experienced industrial pharmacologist with over 20 years of experience gained from pharmaceutical (GlaxoSmithKline) and contract research (BioFocus and Argenta) laboratories. A track record in applied drug discovery, latterly leading and influencing discovery programme teams. Trained initially as an in vitro pharmacologist, Kalpana went on to focus in the field of G-protein coupled receptor drug discovery and specialise in designing and developing plate-based cellular assays to support automated High Throughput Screening and Compound Profiling activities as well as generating mechanism of action follow-up assays. Kalpana joined Essen in 2011, applying her extensive expertise to leading R&D projects developing cellular assays for quantitative live cell analysis.