IncuCyte® Scratch Wound Cell Migration Software Module (Cat. No. 9600-0012)

This software module allows users to automatically process 96-well IncuCyte® scratch wound assay images and to analyze cell migration and invasion without labels. The algorithm masks each phase-contrast image to identify the position of the wounded (cell-free) and unwounded (cell-occupied) zones. Wound width, wound confluence and relative wound density (RWD) metrics are returned, and visualized in time-course plots.

1. Wound Width (μm) - the distance between wound boundaries over time
2. Wound Confluence (%) - cell confluence within the wound region over time. Expressed as the percentage of the wound area occupied by cells.
3. Relative Wound Density (%) - cell density in the wound area expressed relative to the cell density outside of the wound area over time. This metric normalizes for changes in cell density caused by proliferation and/or pharmacological effects.

IncuCyte ZOOM® HD phase contrast image at 14h post wounding of migrating HUVECs. The initial wound ‘mask’ generated with the analysis algorithm is overlaid (blue areas) and defines the wound boundaries at 0h. The wound mask at the 14h time point is shown in yellow.