This software module allows users to automatically process and quantify 96-well IncuCyte® kinetic angiogenesis experiments. Designed to work with our PrimeKit endothelial cell/stromal cell co-culture model, the algorithm masks each fluorescent image to identify and measure the endothelial vascular networks over time. Network length, branch points, area and width uniformity metrics are returned and visualized in time-course plots.
IncuCyte® fluorescent image segmentation of vascular tube formation in a PrimeKit HUVEC and human dermal fibroblast co-cultrue experiment. The image is a blend of the true fluorescent image (green) and the overlaid mask (white). The red lines illustrate the measured tubes.