The IncuCyte® ClearView Chemotaxis Plate combines the throughput of transmembrane assays with the optical clarity of microfluidics devices. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte® Live-Cell Analysis System.
The ClearView membrane contains 96 laser etched pores with a diameter of 8µm. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is visually monitored within your incubator and automatically analyzed using the IncuCyte Live-Cell Analysis System.
Each IncuCyte® ClearView Cell Migration Plate is composed of:
Monitor every cell in your experiment. The ClearView membrane enables label-free visualization and quantitation of chemotaxis using IncuCyte Live-Cell Analysis System. Each membrane contains 96 precision engineered holes in a regular grid array. Three pores are highlighted in yellow.
Lower cell usage. Cell usage comparison for the ClearView Plate and Corning® Transwell® system. Jurkat T-cell chemotaxis toward SDFα-1 was measured in both systems (2,000 to 20,000 cells/well); however, no signal was observed with the Transwell plate. A minimum of 150,000 cells/well was required to observe chemotactic migration using the Corning Transwell system.
Cells must interact with substrate to reach a pore. Time-lapse images of a Jurkat T-cell (outlined in white) migrating across a Matrigel® coating towards a pore leading to the chemoattractant SDF-1α (orange circle). White arrows indicate the direction of Jurkat movement in each frame. In the final image the Jurkat T-cell has entered the pore and moved to the lower chamber.
Long-term stable gradients compared to traditional Boyden chamber transwells. (A) Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran (10 kDa). After 4 hours a rapid loss in gradient was measured in the Corning Transwell system. In contrast, >80% of the gradient was maintained in the IncuCyte ClearView plate at 72 hours. (B) FBS gradients pre-established in a ClearView plate for 24, 48, and 72 hours induced equivalent rates of directional migration in HT1080 cells indicating long-term and stable gradients across the ClearView membrane. Rates were slightly faster than for cells migrating towards gradients that had not been pre-established. This is likely a result of increased cell health when serum starved cells are plated in established gradients.
|ClearView||Boyden Chambers||Microfluidic Devices|
|Visually observe cell
movement and morphology
|Stable chemotactic gradient|
|Low cell usage (< 5,000 per well)|
|Surface interactions– Integrin sensitivity|
|96-well throughput or higher|
|Workflow: set up and walk away|
|Product name||Qty||Catalog No.|
|IncuCyte® ClearView 96-Well Chemotaxis Plate||1 plate||4582|
|IncuCyte® ClearView 96-Well Chemotaxis Plates – Case of 10 Plates||10 plates||4648|
|IncuCyte® ClearView 96-Well Chemotaxis Plates – Case of 50 Plates||50 plates||4599|
|IncuCyte® ClearView Reservoir Plate – Case of 10 Plates||10 plates||4600|
|IncuCyte® ClearView Reservoir Plate – Case of 100 Plates||100 plates||4601|