Reagents & Consumables

One cryopreserved vial of Neuro-2a NucLight Green (nuclear-labelled GFP) expressing cells at a density of 2x10^6 cells/vial.

One cryopreserved vial of HT-1080 NucLight Green and A549 NucLight Red cells (1:1) at a total density of 1x10^6 cells/vial.

One vial of Lentivirus reagent (0.6 mL; >1x10^6 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted red fluorescent label. Stable cell lines can be created under puromycin selection.

One vial of Lentivirus reagent (0.6 mL; >1x10^6 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted green fluorescent label. Stable cell lines can be created under puromycin selection.

One vial of Lentivirus reagent (0.6 mL; >1x10^6 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted red fluorescent label. Stable cell lines can be created under bleomycin selection.

One vial of Lentivirus reagent (0.6 mL; >1x10^6 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted green fluorescent label. Stable cell lines can be created under bleomycin selection.

One vial of lentivirus reagent (0.6mL) suitable for transducing cells with non-perturbing cytoplasmic RFP label. Stable cell lines can be created under puromycin selection.

One vial of lentivirus reagent (0.6mL) suitable for transducing cells with non-perturbing cytoplasmic GFP label. Stable cell lines can be created under puromycin selection.

One vial of lentivirus reagent (0.6mL) suitable for transducing cells with non-perturbing cytoplasmic GFP label.

Sufficient VEGF to supply every well of the 96-well plate at 4 ng/ml (slightly sub-maximal) for the duration of the experiment and enough suramin at 100 µM for 4 wells of a 96-well plate for the duration of the experiment. For use with IncuCyte® Angiogenesis PrimeKit.