Automated phase contrast image segmentation of neurites (yellow) and cell body clusters (blue) in rat cortical neurones using Neurotrack and the IncuCyte® live-cell analysis system.
The IncuCyte® Neurotrack software module allows users to automatically process IncuCyte® images and to analyze neurite dynamics with or without labels in living cells. The algorithm masks and quantifies each image and returns neuronal cell metrics including neurite length, branch points, cell body area and number of cell clusters. These metrics are visualized in full timecourse plots for each well in 96- and 384-well plates. Analyses can be performed directly on phase contrast images obviating the need for cell labels, or on fluorescent images using non-perturbing probes as may be required in co-culture systems.