Incucyte® Neurotrack Analysis Software Module (Cat. No. 9600-0010)

Automated phase contrast image segmentation of neurites

Automated phase contrast image segmentation of neurites (yellow) and cell body clusters (blue) in rat cortical neurones using Neurotrack and the IncuCyte® live-cell analysis system.

The Incucyte® Neurotrack software module allows users to Aautomatically process Incucyte® images and to analyze neurite dynamics with or without labels in living cells in each well of a 96- or 384-well plates. The algorithm masks and quantifies each image and returns neuronal cell metrics including neurite length, branch points, cell body area and number of cell clusters. These metrics are visualized in full timecourse plots for each well in 96- and 384-well plates. Analyses can be performed directly on phase contrast images obviating the need for cell labels in monoculture experiments, or on fluorescent images using non-perturbing probes as may be required inreagents for co-culture systemsmodels.

Automated phase contrast image segmentation of neurites (yellow) and cell body clusters (blue) in rat cortical neuronesneurons using Incucyte® Neurotrack Analysis Software Module and the Incucyte® Live-Cell Analysis System.

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Incucyte® Live Cell Analysis System

Gain insights into biological processes of cells in real time via non-perturbing quantitative analysis

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IncuCyte® NeuroPrime Cell Kit (Cat No 4585)

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat neurons and astrocytes. Supplied as 1 vial (0.45mL) NeuroLight Red Lentiviral Reagent (synapsin promoter), 1 vial (2 x 10^6 cells) IncuCyte® rCortical Neurons (cryopreserved rat cortical neurons), 1 vial (2 x 10^6 cells) IncuCyte® rAstrocytes (cryopreserved rat astrocytes)

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IncuCyte® NeuroLight Red Lentivirus - Synapsin Promoter (Cat No 4807)

One vial of lentivirus reagent (0.45mL) suitable for transducing a variety of neuronal cell types including cell lines, primary and stem cell-derived cultures.

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IncuCyte® ZOOM 20X Objective (Cat No 4465)

20x Microscope Objective for IncuCyte® ZOOM. Required for high spatial resolution cell imaging

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Neuro-2a IncuCyte® NucLight Red Cells (Cat No 4512)

One cryopreserved vial of Neuro-2a NucLight Red (nuclear-labbelled RFP) expressing cells at a density of 2x10^6 cells/vial.

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Neuro-2a IncuCyte® NucLight Green Cells (Cat No 4511)

One cryopreserved vial of Neuro-2a NucLight Green (nuclear-labelled GFP) expressing cells at a density of 2x10^6 cells/vial.

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IncuCyte® rAstrocyte Cells (Cat. No. 4586)

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IncuCyte® NeuroBurst Orange Lentivirus—Synapsin Promoter for Spontaneous Neuronal Activity (Cat No. 4736)

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IncuCyte® rCortical Neurons (Cat. No. 4753)

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IncuCyte® NeuroLight Orange Lentivirus — Synapsin Promoter (cat. No. 4758)

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IncuCyte® Annexin V Orange Reagent for apoptosis (Cat. No. 4759)

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IncuCyte® NeuroPrime Orange Cell Kit (Cat. No. 4760)

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IncuCyte® NeuroActive Orange Cell and Reagent Kit (Cat. No. 4761)

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (2 mL) IncuCyte® NeuroBurst Orange Lentiviral Reagent Cat. No. 4763) (synapsin promoter), 1 vial (2 x 10^6 cells) IncuCyte® rCortical Neurons (cryopreserved rat cortical neurons) (Cat. No. 4753), 1 vial (2 x 10^6 cells) IncuCyte® rAstrocytes (cryopreserved rat astrocytes) (Cat. No. 4586)

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Neurite Dynamics - Label-free

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Neuronal Co-Culture - Fluorescence

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