Live Cell Insights Publications Newsletter
Read our summaries of new and notable research articles featuring the IncuCyte Live Cell Analysis System, including hand-picked publications for applications and research areas like immunotherapy, cell biology, imaging, immune-cell killing, and more!
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IncuCyte has reached 2,000 cited publications spanning a wide array of research areas and applications. We’ve experienced over 50% growth in publications in just the last year! Search our publications list to see what exciting research is being published using the IncuCyte® Live-Cell Analysis System.
Cytotoxic T cells play an immunomodulatory role in controlling human diseases, including cancer. CRISPR-based functional studies in T cells are useful to cancer drug development, but are difficult to carry out in human primary cells. In a joint study, scientists in the laboratory of Dr. Alexander Marson from the University of California, San Francisco, developed a new method, single guide RNA (sgRNA) lentiviral infection with Cas9 protein electroporation (SLICE), which enables loss-of-function pooled screening in primary human T cells at genome-wide scale. Key findings include:
Read the full paper in Cell, November 2018.
FEATURED PRODUCT
IncuCyte® Apoptosis Assays
The mix-and-read IncuCyte® Caspase-3/7 and Annexin V Reagents let you measure multiple apoptotic pathways simultaneously and in real time...
Endoplasmic reticulum (ER) Ca2+ depletion acts as a stress signal to activate the unfolded protein response (UPR), which in turn engages the autophagy and cell death pathways. However, the relationship between these events and the extent and duration of ER Ca2+ depletion is not fully understood. A study led by Dr. Paula Szalaia at the Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership for Molecular Medicine, University of Oslo, Norway, investigated this question by gradually depleting Ca2+ in PC3 cells using sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) inhibitors.
Their data showed that UPR induction, autophagy inhibition or cell death occur in response to extreme Ca2+ depletion. Key findings include:
Read the full paper in Cell Calcium, December 2018.
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IncuCyte® Proliferation Assays
Measure cell proliferation using live cell time-lapse imaging, with or without labels. Easily generate long-term growth and growth-inhibition curves and monitor morphology.
The spinal cord has well-organized neural circuits that sense the environment and produce the appropriate motor response. Despite in vitro data describing motor neuron generation, there are no reports that show induction of the whole spinal cord tissue. In a joint study led by Dr. Takenori Ogura at the Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Japan, and Dr. Hideya Sakaguchi at the Department of Neurosurgery, Kyoto University Graduate School of Medicine, Japan, scientists achieved three dimensional induction of dorsal spinal cord-like tissues using human pluripotent stem cells (hPSCs).
Using this technique, they successfully induced dorsal, intermediate and ventral spinal cord-like tissues. Key findings include:
Read the full paper in Development, July 2018.
FEATURED PRODUCT
IncuCyte® S3 Spheroid Software Module
Analyze growth and shrinkage of spheroids with or without fluorescent label in multi-well formats on the IncuCyte® Live-Cell Analysis System.
Co-administration of anticancer drugs with natural chemotherapeutic agents offers anticancer efficacy with reduced adverse effects. In a joint study between the College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Korea, and the College of Pharmacy, Pusan National University, Korea, scientists designed an oral co-delivery system for IV cancer drug pemetrexed (PMX) and quercetin (QCN), an abundant natural flavonoid.
Their water-in-oil-in-water (w/o/w) nanoemulsion (NE) system showed synergistic efficacy and improved oral absorption in vivo. Key findings include:
Read the full paper in Pharmaceutics, September 2018.
FEATURED PRODUCT
IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Reagent couples the activated caspase-3/7 recognition motif (DEVD) to NucView™ 488, a DNA intercalating dye to enable quantification of apoptosis over time.
EgKI-1 protease is highly expressed by the infectious oncosphere stage of the canine tapeworm Echinococcus granulosus, which causes hydatid cysts in human organs. Larval protoscoleces inside the hydatid cysts have demonstrated killing of some tumors via an unknown mechanism. A study led by Dr. Shiwanthi Ranasinghe at the Molecular Parasitology Laboratory, Immunology Department, QIMR Berghofer Medical Research Institute, Australia, showed antitumor activity of recombinant EgKI-1 protease in several cancer cell lines. They also found in vivo evidence of tumor growth inhibition in an EgKI-1-treated breast cancer mouse model. Key findings include:
Read the full paper in PLoS One, August 2018.
FEATURED PRODUCT
IncuCyte® Cell Migration Kit
Comprises everything you need to run fully kinetic and automated 96-well cell migration and invasion scratch wound assay.
Alizarin Red is traditionally used to detect mineralization as a marker of osteoblast differentiation. However, the requirement of cell fixation limits this method to one time point per sample. Dr. Anastassia Serguienko at the Institute for Cancer Research, Oslo University Hospital, Norway, and colleagues, addressed this technical gap by developing a live-cell imaging methodology using Calcein Green.
They combined Calcein Green and the IncuCyte® system as a live-cell imaging tool for monitoring the dynamics of matrix mineralization. Key findings include:
Read the full paper in Methodology, August 2018.
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte® S3 Live-Cell Analysis System automatically acquires and analyzes images around the clock, providing information-rich analysis that is easy to achieve.
Mebendazole (MBZ), used to treat helminthic diseases, has demonstrated anticancer activity and induced a proinflammatory tumour-suppressive M1 phenotype in THP-1 monocytes and macrophages. Dr. Jenny Rubin and colleagues at the departments of Medical Sciences and Immunology, Uppsala University, Sweden, used human peripheral blood mononuclear cells (PBMCs) co-cultured with tumor cells to understand the immune modulating and anticancer properties of MBZ.
They found that MBZ potentiated the immune stimulatory and anticancer activity of CD3/IL2-activated PBMCs and that this effect was dependent on the presence of attenuated CD14 positive cells. Key findings include:
Read the full paper in Oncotarget, July 2018.
FEATURED PRODUCT
IncuCyte® Immune Cell Killing Assays
An integrated solution for real-time visualization and automated analysis of immune cell-mediated killing of tumor cells – all within your tissue culture incubator...
Systemic inflammation is believed to cause an increased risk of cardiovascular disease (CVD) in human immunodeficiency virus (HIV) patients, but the mechanism remains unknown. A study lead by Dr. Samir Gupta at the Indiana University School of Medicine, Indianapolis, used a new flow cytometry assay to study how monocyte activation leads to CVD in HIV patients, including those receiving suppressive antiretroviral therapy (ART).
The data showed that monocyte activation in HIV-infected individuals adversely affects the functional parameters of endothelial progenitor cells, “endothelial colony-forming cells” (ECFCs), which have angiogenetic properties. Key findings include:
Read the full paper in The Journal of Infectious Diseases, September 2018.
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte® S3 Live-Cell Analysis System automatically acquires and analyzes images around the clock, providing information-rich analysis that is easy to achieve.