Live Cell Insights Publications Newsletter
Read our summaries of new and notable research articles featuring the IncuCyte Live Cell Analysis System, including hand-picked publications for applications and research areas like immunology, oncology, neurology, and more!
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IncuCyte has almost reached 2,000 cited publications spanning a wide array of research areas and applications. We’ve experienced over 50% growth in publications in just the last year! Search our publications list to see what exciting research is being published using the IncuCyte® Live-Cell Analysis System.
Neural crest migration is vital to the function of neural crest cells, and its misregulation is associated with cancer. However, many questions remain about the mechanisms that control its vital migratory activity. Scientists from the Centre for Craniofacial and Regenerative Biology, King’s College London, studied the effect of the serine/threonine kinase GSK3 — previously shown to play an important role in craniofacial development — in neural crest migration in mouse and Xenopus.
Their studies show that GSK3 is tyrosine phosphorylated in migratory neural crest (mNC) cells, and its activity is under the control of a protein associated with neuroblastoma. Their findings include:
Read the full paper in Nature Communications, March 2018.
FEATURED PRODUCT
IncuCyte® Scratch Wound Cell Migration Assay
The IncuCyte® Scratch Wound Cell Migration Assay enables real-time visualization and assessment of cell morphology in scratch wound assays from inside your tissue culture incubator.
Current efforts to develop an anti-Brucella vaccine focus on safer approaches that utilize inactive, subunit vaccines and vectors that avoid introducing the complete Brucella organism. Scientists led by Dr. Lalsiamthara at the Chonbuk National University, Iksan Campus, in the Republic of Korea, previously developed an anti-Brucella vaccine candidate based on a Salmonella Typhimurium vector cocktail delivering immunogenic Brucella proteins superoxide dismutase (SOD), Brucella lumazine synthase (BLS), outer membrane protein-19 (OMP19) and proline racemase protein A (prpA). This study assesses the lethal dose in mice and toxicity to cultured human cells.
The data show high safety in mouse models and good safety profile in cultured human cells. They found that:
Read the full paper in Vaccine, March 2018.
FEATURED PRODUCT
IncuCyte® Cytotox Green Reagent
IncuCyte® Cytotox Reagents are highly sensitive cyanine nucleic acid dyes for a simple mix-and-read, real-time quantification of cell death.
The cell-surface coreceptor Neuropilin-1 (NRP1) binds a number of different growths factors, including isoforms of vascular endothelial growth factor (VEGF). Given the role of the immune system in cancer progression, NRP1 antagonists are desirable candidates for development as anticancer drugs.
In a joint study between the Institute of Structural and Molecular Biology, University College London, and the Department of Pharmacology, Stony Brook University, in New York, scientists used a previously published NRP1/VEGF-A antagonist (S)-2-(3-(benzo[c][1,2,5]thiadiazole-4-sulfonamido)-thiophene-2-carboxamido)-5-((diaminomethylene)amino)-pentanoic acid (EG00229) as a starting point to develop better candidates. They identified a new lead 1 (EG013777) and demonstrated antimigratory and antitumor activity in preliminary studies.
Read the full paper in J Med Chem, May 2018.
FEATURED PRODUCT
IncuCyte® Scratch Wound Cell Migration Assay
The IncuCyte® Scratch Wound Cell Migration Assay enables real-time visualization and assessment of cell morphology in scratch wound assays from inside your tissue culture incubator.
Extracellular histone release in patients with disseminated intravascular coagulation (DIC) causes platelet aggregation, neutrophil migration, and lethal thrombosis. Linear polysaccharide heparin binds negatively to charged histones, but it also blocks the coagulation pathway. Chondroitin sulfate (CS) has been used as a nutraceutical and a drug for osteoarthritis and is less inhibitory to the coagulation pathway. A study led by Dr. Nagano at the Department of Analytical Pharmacology, Meijo University, in Japan, investigated the interaction between CS and extracellular histones as a new strategy to treat lethal thrombosis induced by histones.
The data supports using CS as a novel agent to treat lethal thrombosis in DIC patients. Key finds are:
Read the full paper in Eur J Pharmacol, May 2018.
FEATURED PRODUCT
IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent offers a no wash, mix and read solution to enable quantification of apoptosis over time of cells undergoing caspase-3/7 mediated apoptosis.
Cancer-associated fibroblasts (CAFs) secrete a variety of factors and extracellular vehicles (EVs) that promote tumor growth. Dr. Principe and colleagues at the Princess Margaret Cancer Centre in Canada embarked on a proteomics approach to better understand the role of CAFs in oral tongue squamous cell carcinoma (OTSCC) and to identify novel CAF associated biomarkers.
They identified 4247 proteins, including microfibrillar-associated protein 5 (MFAP5) as a novel CAF-enriched protein. Their report shows:
Read the full paper in J Proteome Res, June 2018.
FEATURED PRODUCT
IncuCyte® Proliferation Assays
Measure cell proliferation using live cell time-lapse imaging, with or without labels. Easily generate long-term growth and growth-inhibition curves and monitor morphology.