Live Cell Insights Publications Newsletter
Read our summaries of new and notable research articles featuring the IncuCyte® Live Cell Analysis System, including hand-picked publications for applications and research areas like immunology, oncology, tumor spheroids, and more!
Don't miss out on new and notable IncuCyte® publications! Be the first to see IncuCyte Live-Cell Analysis System in peer-reviewed journals.
Let us know how we can help you get your research published using the IncuCyte!
IncuCyte has now reached over 1,500 cited publications spanning a wide array of research areas and applications. We’ve experienced over 50% growth in publications in just the last year! Search our publications list to see what exciting research is being published using the IncuCyte® Live-Cell Analysis System.
The root cause of neurodegenerative disorders is often the accumulation of aggregated, misfolded proteins. As a novel approach to identifying druggable targets for neurodegenerative diseases, scientists from Fudan University in Shanghai capitalized on the monogenetic nature of Huntington’s Disease (HD) that is caused by accumulation of mHTT protein produced by the mutant Huntingtin gene as a simple model system for screening for modulators of mHTT protein levels.
Their studies identified MAPK11 and HIPK3 kinases as novel modulators of mHTT levels and confirmed their potential as therapeutic targets for HD both in vitro and in vivo. Their findings show:
Read the full paper in Cell Res, December 2017.
FEATURED PRODUCT
IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent offers a no wash, mix and read solution to enable quantification of apoptosis over time of cells undergoing caspase-3/7 mediated apoptosis.
To infect cells and cause disease, viruses traverse the endocytic network to transport viral particles to their specific cellular destination. Along the way, viruses exploit the endocytic environment and maturation of the endocytic vesicles to trigger the expulsion of its genetic material into the cytosol. This process is regulated by various factors, including pH, proteolysis, and lipid composition.
Scientists from the laboratory of Dr. Jamel Mankouri at the University of Leeds in the United Kingdom had previously identified K+ channels as yet another critical factor in trafficking of the prototypic bunyavirus, orthobunyavirus Bunyamwera virus (BUNV) through the endocytic network. Here, the researchers sought to determine why K+ channels are critical for BUNV progression through the endocytic network and found:
Read the full paper in PloS Pathogens, January 2018.
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock – right inside your incubator, providing information-rich analysis that is easy to achieve.
To gain a better understanding of cell-cell interactions in the tumor microenvironment and how they influence tumor motility and dissemination, a collaborative research effort led by Dr. Cecilia Moens at Fred Hutchinson Cancer Research Center sought to capture the dynamics that occur in interactions between tumor cells and tumor-associated macrophages using high-resolution imaging of in vivo model systems.
Tumor-associated macrophages undergo a phenotypic switch through signaling in the tumor microenvironment, leading them to promote tumorigenesis and be associated with poor prognosis. Researchers sought to improve current end-point and in vitro methods to study macrophage interaction with tumor cells by utilizing in vivo zebrafish xenotransplant and mouse models to study the melanoma tumor microenvironment. These studies uncovered:
Read the full paper in Developmental Cell, December 2017.
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock – right inside your incubator, providing information-rich analysis that is easy to achieve.
Conventional monolayer cell cultures on non-biological rigid surfaces lack multiple aspects of the in vivo cellular environment including regulatory cues from extracellular matrix (ECM) components. 3D scaffold culture models incorporating purified ECM components offer a more physiological growth environment than 2D models but do not fully recapitulate the protein complexity of the in vivo ECM. Cell-derived matrices (CDM) consist of naturally secreted ECM components and closely reproduce the physiological properties of the cellular microenvironment.
Researchers at the University of Turku, Finland, report a reproducible method for CDM production from fibroblasts and illustrate applications of CDMs as follows:
Read the full paper in Nature Protocols, November 2017.
FEATURED PRODUCT
IncuCyte® NucLight Rapid Red Reagent for live-cell nuclear labeling
The IncuCyte Rapid Red Reagent for cell labeling is a cell permeable DNA stain that specifically stains nuclei in live cells and is ideally suited to the mix-and-read, real-time quantification of cell counting.
Photodynamic therapy (PDT) is a non-systemic cancer treatment utilizing photosensitizing drugs in combination with specific light exposure. PDT with proto-porphyrin IX precursors, which are preferentially metabolized by hyperactive cells, has applications in urology, gastroenterology and dermatology to kill precancerous and malignant cells, but is ineffective against melanomas and more advanced cancers.
Hematoporphyrin-derived photosensitizers such as 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide (HPPH) may be effective for PDT of invasive cancers, but effective targeted delivery approaches preventing collateral damage to healthy tissues are lacking. Investigators at the Singapore Agency for Science, Technology and Research now report the development of a peptide-ligated photosensitizer with specificity for melanocytes and melanoma that spares surrounding keratinocytes. Their report shows:
Read the full paper in Scientific Reports, November 2017.
FEATURED PRODUCT
IncuCyte® Proliferation Assays
Measure cell proliferation using live cell time-lapse imaging, with or without labels. Easily generate long-term growth and growth-inhibition curves and monitor morphology.
KRAS is one of the most frequently mutated oncogenes detected in cancers, including lung adenocarcinoma (LUAD), and can result in constitutively activated MAPK survival signaling in tumor cells. KRAS mutant cancers are typically resistant to targeted therapies such as RAF/MEK/ERK inhibition and don’t respond well to standard of care chemotherapy so the need for effective treatment is urgent. This study looks to understand the critical role of wild-type KRAS and mutant KRAS dimerization in tumor fitness and resistance to MEK inhibitors, which are used to disrupt the MAPK signaling pathway that promotes tumor cell proliferation, angiogenesis, and resistance to apoptosis.
A collaboration between researchers from Dana Farber Cancer Institute and other institutions and working with a KRAS loss of heterozygosity (LOH) model that is genetically inducible, and a dimerization-deficient (charge-reversal) D154Q mutant KRAS, at the alpha4-alpha5 KRAS dimer interface, interrogated the role of KRAS dimerization, both in vitro and in vivo, in KRAS-mutant cancers, uncovering new insights that reveal a potential therapeutic strategy for further exploration. These studies show:
Read the full paper in Cell, February 2018.
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock – right inside your incubator, providing information-rich analysis that is easy to achieve.