Live Cell Insights Publications Newsletter
Read our summaries of new and notable research articles featuring the IncuCyte® Live Cell Analysis System, including hand-picked publications for applications and research areas like immunology, oncology, tumor spheroids, and more!
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Necroptosis is defined as an “immunogenic” cell death and is a form of regulated necrosis governed by the RIPK3-mediated activation of MLKL pseudokinase which induces disruption of the plasma membrane. The components and signals that regulate necroptosis activity are currently under investigation. In this study, Gong et al. examined the mechanism by which ESCRT-III antagonizes necroptosis. They found the following:
Read the full paper in Cell, April 2017
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IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock – right inside your incubator, providing information-rich analysis that is easy to achieve.
Unlike apoptosis, necroptosis and pyroptosis represent ‘alternative forms’ of lytic programmed cell death. Pyroptosis is triggered by inflammasome-mediated activation of the inflammatory caspases that promotes inflammation through caspase-mediated processing of cytokines IL-1β and IL-18.
In studies of the inherent mechanistic link between the execution of the necroptotic program inflammasome activation and caspase-mediated processing of cytokines, a team lead by Andrew Oberst at the Center for Innate Immunity and Immune Disease, University of Washington, Seattle, demonstrated:
Read the full paper in The Journal of Immunology, March 2017
FEATURED PRODUCT
IncuCyte® S3 Live-Cell Analysis System
The IncuCyte automatically acquires and analyzes images around the clock – right inside your incubator, providing information-rich analysis that is easy to achieve.
Targeted therapies to the BRAF/MEK/ERK pathway have had a significant impact on prognosis and survival of patients with advanced melanoma. However, off-target effects and acquired resistance are often observed in these patients, warranting further studies to gain a better understanding of melanoma response to inhibitors of the BRAF/MEK/ERK pathway in order to develop more effective treatments for melanoma.
In the current study, researchers from Poland utilized six melanoma cell populations harboring an activating mutation in BRAF (V600E) to characterize their response to vemurafenib (PLX) and trametinib (TRA), two FDA approved drugs for the treatment of advanced melanoma, at both the cellular and molecular level. The authors found that:
Read the full paper in Laboratory Investigation, February 2016
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IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent
The IncuCyte® Caspase-3/7 Green Apoptosis Assay Reagent offers a no wash, mix and read solution to enable quantification of apoptosis over time of cells undergoing caspase-3/7 mediated apoptosis.
In vitro spheroid tumor models are being developed that better mimic the in vivo physiological context of tumors, leading to more efficient discovery of drugs and drug targets, and predictive pharmacological responses.
Here, scientists from the laboratory of Dr. Marc Ferrer at the NIH describe the development of a real-time tumor spheroid formation, HTS-compatible screening assay platform in order to provide a physiologically relevant cell model system to bridge the gap between monolayer model systems and costly clinical studies. 11 different chemotherapeutic compounds were tested in a dose respondent manner, and GFP expression was continuously measured in Hey-A8-GFP cells. The researchers reported the following:
Read the full paper in SLAS Discovery, March 2017
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IncuCyte® CytoLight Green Lentivirus (EF-1 Alpha Promoter, Puromycin selection)
Specially designed to efficiently transduce multiple cell types and provide homogenous expression with non-perturbing cytoplasmic GFP label. Stable cell lines can be created under puromycin selection.
The wound healing properties of adipose-derived stem cells (ASCs) have been demonstrated in pre-clinical studies, as ASCs secrete soluble factors which promote angiogenesis, keratinocyte re-epithelization, and protect against apoptosis. However, to harness their potential for use in regenerative therapies, more effective in vitro models are needed to study the various aspects of ASC wound healing properties. The laboratory group of Dr. Trine Fink at Aalborg University in Denmark demonstrate that ASC-keratinocyte scratch assays can be modified to better mimic in vivo re-epithelization and model wound healing properties of ASCs. Their findings show that:
Read the full paper in International Journal of Molecular Medicine. February 2017
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IncuCyte ® Woundmaker, Software Module and Cell Migration Kit
An integrated solution for real-time visualization and assessment of cell morphology in scratch wound assay, up to six 96-well plates at once – all inside your incubator.