To develop and validate next generation immuno-oncology therapies researchers have a need to study the basic functions of tumor and immune cells in isolation and together.
Common assays for measuring key functions such as In vitro tumor cell proliferation, migration and invasion are often end point, require cell lifting or measuring indirect readouts. As a result, valuable data may be overlooked or compromised. These assays provide little or no information around the time course of events and are unable to reveal the dynamic interactions between cells.
The IncuCyte® Live-Cell Analysis System enables real-time, automated and direct measurements of the dynamic interactions between immune and cancer cells. The system provides non-invasive measurements so that cells or supernatants can be used for downstream assays or further complementary analyses (e.g. flow cytometry, biochemical analysis).
Apoptosis is also known as Programmed Cell Death, it is an important process studied by cell biologists in cancer, immunology and neuroscience where a cell in response to some stimuli induces a cell death cascade
Incucyte™ apoptosis assays detect apoptosis in living cells and in real time using simple mix-and-read protocols.
The phagocytosis of diseased or dying cells is a key function of phagocytic immune cells such as macrophages. The clearance of dead or apoptotic cells by phagocytosis is called efferocytosis and is critical to resolving episodes of inflammation (e.g. removal of neutrophils that persist following inflammation) and preventing the development of chronic inflammatory or autoimmune diseases (e.g., COPD, asthma, rheumatoid arthritis, fibrosis, and atherosclerosis). Efferocytosis is also key for the efficient removal of apoptotic tumor cells following treatment with anti-cancer drugs ensuring that cellular contents are contained and circumventing unwanted inflammatory responses.
The IncuCyte® Live-Cell Analysis system enables real-time, automated cellular phagocytosis and effrocytosis assays within your cell culture incubator.
This updated version for the IncuCyte ZOOM® Live-Cell Imaging system introduces Coincident Object Analysis (COA) to provide enhanced user-friendly control of all aspects of imaging and analysis.
Coincident Object Analysis (COA) Automatically quantify red and green fluorescence overlap. Selectively analyze co-labelled objects and measure changes in fluorescence coincidence over time. The IncuCyte™ Coincident Object Analysis tool enables calculation of the total number, and area, of overlapping objects. Example applications include:
Over 800 peer-reviewed publications* in research areas including:
*Publications may appear in more than one research category.
Llambi, F. et al. BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
Cell (2016) April 7;165(2):421-33
Zhou, Q. et al. Topoisomerase IIα mediates TCF-dependent epithelial-mesenchymal transition in colon cancer.
Oncogene (2016) March 7
Bian, M. et al. Celastrol protects mouse retinas from bright light-induced degeneration through inhibition of oxidative stress and inflammation.
J Neuroinflammation (2016) February 27;13:50
Shah, ET. et al. Repositioning old drugs for new causes: identifying new inhibitors of prostate cancer cell migration and invasion.
Clin Exp Metastasis (2016) April:33(4):385-99
Marklein, RA. et al. High content imaging of early morphological signatures predicts long term mineralization capacity of human mesenchymal stem cells upon osteogenic induction.
Stem Cells (2016) February 11.
Pasqualon, Tet. et al. Cell surface syndecan-1 contributes to binding and function of macrophage migration inhibitory factor (MIF) on epithelial tumor cells.
Biochim. Biophys. Acta (2016) April;1863(4):717-26
Vakhrushev, IV. et al. Variability of the Phenotype and Proliferation and Migration Characteristics of Human Mesenchymal Stromal Cells Derived from the Deciduous Teeth Pulp of Different Donors.
Bull. Exp. Biol. Med. (2016) February;160(4):525-9
Bachstetter, AD. et al. MW151 Inhibited IL-1β Levels after Traumatic Brain Injury with No Effect on Microglia Physiological Responses.
PLoS ONE (2016) February 12.
Yamada, K. et al. Impaired nuclear factor erythroid 2-related factor 2 expression increases apoptosis of airway epithelial cells in patients with chronic obstructive pulmonary disease due to cigarette smoking.
BMC Pulm Med. (2016) February 9;16(1):27
INCUCYTE™ TIPS & TRICKS
IncuCyte Scratch Wound assays deliver optimal performance when cells approach 100% confluence right at the time of wounding. However, the number of cells necessary to achieve this confluency varies depending on cell line. To determine the optimal seeding density, simply seed your cells at a range of different densities in ImageLock plates. 10K-50K cells per well is a common range for testing. Image your cells for 6-18 hours and set up a confluence analysis job using the Zoom software. Graph your data using the % confluence metric and identify the minimum seeding density necessary to achieve the desired confluence at wounding.
You can also use the images to confirm the best seeding density. Cells which are too dense will peel off in sheets post-scratch. You’ll be able to use this seeding density in all future scratch wound assays for this cell line! Share your tips & tricks in using your IncuCyte™ instrument!
ASK AN EXPERT
A: You must archive and delete scans to free up space to continue scanning. Note that once data is archived it cannot be re-analyzed again. To initiate archiving, select the Archives option located on the left side task list. Once selected, the “Create New Archive” table is active, displaying all vessels available for archiving. Users can search the entire Archiving Vessels table using the “Find” field or within individual columns.
Once the vessels of interest are sorted (e.g. by date or by project), Users may select vessels individually or use the “Select All” button located in the lower left-corner of the window. Select the “Create Archive” button in the lower right-corner of the window and you will be asked to specify the archive destination. Users have the option to select either “My Computer” or “Storage Attached to ZOOM”. Depending on how large the archive is, it could take hours, or even days. It’s best to archive in smaller chunks then a huge TB+ size at a time.
After archiving, you can delete those scans to free up more space. Select the Admin option on the left side task list, and then select the Delete tab. Once selected, the table is active displaying all scans available for deleting. Select the scans you previous archived for delete and click Delete.