Cell health and viability assays enable the measurements of cell health, which are essential when studying the effects of drugs, culture conditions or genetic modifications on cell growth or viability. These assays also provide crucial means for ranking compounds in drug discovery screens and enable investigation into the cellular changes that underlie disease pathologies.
Many existing methods for assessing cell health and viability rely on indirect, end-point measurements that are subject to artefacts and cannot be readily verified by morphology changes. IncuCyte™ Cell health and viability assays provide direct, image-based cell health measurements in real time within a standard cell culture incubator.
The NucLight™ BacMam 3.0 reagents are used for non-perturbing, nuclear labeling of living mammalian cells. Using a simple mix-and-read protocol, these reagents enable real-time cell counting within hours of infection, thus allowing calculations of doubling times and direct measures of cellular proliferation. The reagents are optimized for the IncuCyte® system - you don’t need to worry about compatibility.
The IncuCyte™ Cytotoxicity Assays measure cell death based on cell membrane integrity in real time, all within your incubator. Using a simple mix-and-read protocol, these reagents enable real-time quantification of cell death. The reagents are optimized for the IncuCyte® system – you don’t need to worry about compatibility.
IncuCyte™ NucLight™ Reagents enable efficient nuclear labeling and counting of living cells without perturbing cell function or morphology. They are a unique range of BacMam and Lentiviral-based labeling reagents with simple transduction protocols that enable expression of a nuclear-restricted green (GFP) or red (mKate2) fluorescent protein in your choice of primary, immortalized, dividing, or non-dividing cells.
IncuCyte™ CytoLight™ Reagents enable efficient cytoplasmic labeling of living cells and monitoring cell-cell interactions without perturbing function or morphology. They are a unique range of lentiviral-based labeling reagents with simple transduction protocols that measure cell health in 3D spheroids and enable expression of a cytoplasmically soluble green (GFP) or red (mKate2) fluorescent protein in your choice of primary, immortalized, dividing, or non-dividing cells.
Over 700 peer-reviewed publications* in research areas including:
*Publications may appear in more than one research category.
1. Park, S.H. et al. Vascular Protective Effect of an Ethanol Extract of Camellia japonica Fruit: Endothelium-Dependent Relaxation of Coronary Artery and Reduction of Smooth Muscle Cell Migration. Oxid. Med. Cell. Longev. 2016, 1–9 (2016).
2. Neri, S. et al. Cancer cell invasion driven by extracellular matrix remodeling is dependent on the properties of cancer-associated fibroblasts. J. Cancer Res. Clin. Oncol. 142, 437–446 (2016).
3. Karnezis, A. N. et al. Dual loss of the SWI/SNF complex ATPases SMARCA4/BRG1 and SMARCA2/BRM is highly sensitive and specific for small cell carcinoma of the ovary, hypercalcaemic type. J. Pathol. 238, 389–400 (2016).
4. Jamshidi, F. et al. The genomic landscape of epithelioid sarcoma cell lines and tumours. J. Pathol. 238, 63–73 (2016).
Experimental data is influenced by many factors including initial growth conditions, cell counting, and dissociation methods. In order to improve cell performance we recommend the following techniques:
1) Prior to assay initiation maintain cell culture at or below 80% confluence.
2) Ensure that you have accurate cell counts by thoroughly mixing your cells prior to sampling.
3) Limit the time the cells are exposed to enzymatic dissociation.
A: The growth curve of each flask must be generated separately, creating two separate graphs. For their direct growth comparison you can combine both graphs into one by selecting “Graph” on Graph Option in the Drag and Drop box at the bottom of the graph window. Simply right-click on, and hold the graph to be dragged, and then use the mouse to drag it onto the target graph.
Release of the mouse button will cause the graph to be dropped. The new graph will still contain the title from the original, Growth Curve for Vessel 1- however, it will now contain two traces labeled as Vessel 1 and Vessel 2.
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