In This Issue

Just Released

Immune Cell Killing, Clustering & Proliferation
IncuCyte™ Chemotaxis Cell Migration Assay
New IncuCyte™ Software Release

Publication Updates

In every issue, learn how researchers around the world are using IncuCyte™ systems across a range of applications and research areas. Read publications now

IncuCyte™ Tips and Tricks

Ask an Expert

Find answers to questions submitted by readers just like you. See latest answers

Discounts & Promotions

Get updates on our latest discounts and promotions. See our newest discounts and promotions

Upcoming events

Find out what's happening near you. See latest events


JUST RELEASED

Immune Cell Killing, Clustering & Proliferation

What is Immune Cell Killing?

Immune-cell recognition and the targeting of harmful cells, such as cancer, is a critical component of our human host defense mechanism. Cytotoxic cell killing, also known as “T cell killing” is a mechanism of our immune response.

What is our solution?

The IncuCyte™ Immune Cell Killing Assay is an integrated solution for real-time visualization and automated analysis of T cell killing– all within your tissue culture incubator.

What it offers

  • Real-time visualization and fully automated analysis of tumor cell killing
  • Direct measurements of tumor cell death and viability
  • Make relevant measurements in a highly flexible assay format
  • Mix-and-read 96/384-well assays suitable for screening

Learn more about Immune Cell Killing


What is Immune Cell Clustering and Proliferation?

During an immune response, specific cells of the immune system, such as T lymphocytes, change dynamically in order to fight infection or disease. These changes are fundamental to regulating the effect and extent of the immune response.

What is our solution?

The IncuCyte™ immune cell clustering & proliferation assays allow visualization and quantification of immune cell interactions and proliferation in real time. Explore the mechanisms of proliferation, regulation and clustering within your incubator and without the need to lift or label your cells.

What it offers

  • Real-time visualization and automated analysis
  • Monitor the full time course within your cell incubator
  • Label-free, kinetic measurements
  • No washing, no cell lifting, no antibody labeling

Learn more about Immune Cell Clustering and Proliferation

IncuCyte™ Chemotaxis Cell Migration Assay

What is chemotaxis?

Chemotaxis is the directional movement of cells in response to a chemical stimulus, and is an essential component of immune responses, tumor metastasis, wound healing and blood vessel formation.

What is our solution?

An integrated solution for real-time visualization and automated analysis of chemotactic cell migration for label-free or fluorescently labeled cells in a 96-well format – all within your tissue culture incubator.

What it offers

  • See morphological changes, cell-cell interactions and collective cell migration with high definition images
  • Set up experiment and walk away with no post-labeling, scraping or need to pre-define the assay endpoint
  • Low cell usage, ideal for rare, expensive and primary cell types

Learn more about IncuCyte™ Chemotaxis Cell Migration Assay

New IncuCyte™ ZOOM Software (2015A):

What is our product?

This upgraded version for the IncuCyte ZOOM® Live-Cell Imaging system provides key enhancements for performing assays.

What it offers

  • Added capability of 384-well whole-well imaging
  • Enhanced NeuroTrack™ fluorescence tools
  • New fluorescence analysis tools for co-culture scratch wound assays
  • Improved scheduling flexibility using the new Restore feature

Upgrade is free for units currently under warranty.

Learn more about the new IncuCyte™ ZOOM software

PUBLICATION UPDATES

Publication Updates from the IncuCyte™ Community

The number of IncuCyte™ references has now surpassed 500! Below are three highlights from July, 2015.

Inhibition of endogenous hydrogen sulfide production in clear-cell renal cell carcinoma cell lines and xenografts restricts their growth, survival and angiogenic potential Sonke E,Verrydt M,Postenka CO,Pardhan S,Willie CJ,Mazzola CR,Hammers MD,Pluth MD,Lobb I,Power NE,Chambers AF,Leong HS,Sener A,
Nitric Oxide, 49:26-39, 2015

Real-time monitoring of the hypoxic marker, hydrogen sulfide, using IncuCyte ZOOM® reveals novel therapeutic opportunities in the fight against renal cell carcinoma.

Targeting the podocyte to treat glomerular kidney disease
Lal MA,Young KW,Andag U,
Drug Discov. Today, pii: S1359-6446(15)00233-0, 2015

Researchers at AstraZeneca and Evotec use IncuCyte™ to functionally validate and assess the potency of screening hits in an ex vivo model of diabetic nephropathy revealing novel opportunities for kidney-specific therapeutics.

The HDAC Inhibitors Scriptaid and LBH589 Combined with the Oncolytic Virus Delta24-RGD Exert Enhanced Anti-Tumor Efficacy in Patient-Derived Glioblastoma Cells Pont LMEB,Kleijn A,Kloezeman JJ,
PLoS ONE, 10, e0127058, 2015

Enhancing oncolytic therapy: IncuCyte™ real-time monitoring of viral replication in patient-derived glioblastoma stem-like cells identify co-treatments that augment viral infection and cause synergistic anti-tumor activity.

Publications Map

The number of IncuCyte™ references has surpassed 500! View the publications in each country and see which research institutions are using IncuCyte™ live-cell imaging.


INCUCYTE™ TIPS & TRICKS

Seeing bubbles in your images?

If the bubble is on top of your cell culture media, ethanol vapor is a great way to rid yourself of them. Simply take an ethanol squeeze bottle, remove the internal straw and gently squeeze the bottle, while moving the tip across your plate, effectively blowing ethanol vapor across the surface of your media and ridding yourself of pesky top-side bubbles. We at Essen BioScience call these handy bottles “Debubblers”!

Share your tips & tricks in using your IncuCyte!


ASK AN EXPERT

Q: Our system indicates a “Disk Nearly Full” or “Disk Full” status in the software or on the touch screen LCD panel, what should we do?

A: You must archive and delete scans to free up space to continue scanning. Note that once data is archived it cannot be re-analyzed again. To initiate archiving, select the Archives option located on the left side task list. Once selected, the “Create New Archive” table is active, displaying all vessels available for archiving. Users can search the entire Archiving Vessels table using the “Find” field or within individual columns.

Once the vessels of interest are sorted (e.g. by date or by project), Users may select vessels individually or use the “Select All” button located in the lower left-corner of the window. Select the “Create Archive” button in the lower right-corner of the window and you will be asked to specify the archive destination. Users have the option to select either “My Computer” or “Storage Attached to ZOOM”. Depending on how large the archive is, it could take hours, or even days. It’s best to archive in smaller chunks then a huge TB+ size at a time.

After archiving, you can delete those scans to free up more space. Select the Admin option on the left side task list, and then select the Delete tab. Once selected, the table is active displaying all scans available for deleting. Select the scans you previous archived for delete and click Delete.

Have a technical support question?

Ask our technical support team!


DISCOUNTS & PROMOTIONS

New Lab Startup

Our New Lab Startup Program is a program designed to help you get your new lab up and running. The program connects you with exclusive discounts and resources such as a discount on your first IncuCyte™ system order.

To verify your eligibility, please visit our website and register.

Learn More about the New Lab Startup Program

IncuCyte™ Referral Program

Our IncuCyte™ Referral Program is a program that rewards you when someone you refer to us, purchases an IncuCyte™ system.

Learn More about the IncuCyte™ Referral Program

Find us on Facebook

4,000 fans and growing! “Like” us and stay connected!

Connect with us on Facebook now


UPCOMING EVENTS

September:

  • 09/02-03 2015 9th Annual ELRIG Drug Discovery, Telford, United Kingdom
  • 09/10-11, 2015 6th Annual Meeting - Industrial Cell Technology Symposium, Lübeck, Germany
  • 09/22-24, 2015. MipTec 2015, Basel, Switzerland

October:

  • 10/06 2015 Journée découverte, Paris, France
  • 10/08-10, 2015 The 74th Annual Meeting of the Japanese Cancer Association, Nagoya, Japan
  • 10/17-21, 2015 Society for Neuroscience Annual Meeting, Chicago, USA

November:

  • 11/18-20, 2015 The 44th Annual Meeting of The Japanese Society for Immunology, Exhibition, Sapporo, Japan
  • 11/26, 2015 Incucyte™ Live Cell Analysis Seminar, Tokyo, Japan

December:

  • 12/01-04, 2015 Biochemistry and Molecular Biology 2015, Kobe, Japan
  • Dec 13-15. American Society for Cell Biology 2015, San Diego, USA
No file selected
0%
Stay up-to-date with what's happening at Essen BioScience

© 2017 Essen BioScience

Privacy Policy