Cell migration is a fundamental component of many biological processes including embryonic development, tissue re-organization, angiogenesis and cancer metastasis.
Monitor and measure cell migration across coated or uncoated surfaces in real time using the IncuCyte™ scratch wound assay and the Cell Migration Kit (Cat No 4493).
Cell invasion is the movement of cells through a three-dimensional extracellular matrix and is essential in normal cells, such as inflammatory response of leukocytes, as well as tumor metastasis.
Image and quantify cell invasion through 3D bio matrix gels in real time using the IncuCyte™ scratch wound assays, Cell Migration Kit (Cat No 4493) and Cell Invasion Accessories (Cat No 4444).
Nearly 600 peer reviewed publications in research areas including:
Publications may appear in more than one research category.
1. MYC Is a Major Determinant of Mitotic Cell Fate. Topham, C, Tighe, A, Ly, P, Bennett, A, Sloss, O, Nelson, L, Ridgway, RA, Huels, D, Littler, S, Schandl, C, Sun, Y, Bechi, B, Procter, DJ, Sansom, OJ, Cleveland, DW, Taylor, SS. Cancer Cell 28, 129–140 (2015).
Inhibition of the oncogenic transcription factor Myc attenuates apoptosis in response to a panel eight antimitotic agents across a range of colon, lung, cervical, breast and ovarian cancer cell lines.
2. Heat-shock factor 2 is a suppressor of prostate cancer invasion. Björk, JK, Ĺkerfelt, M, Joutsen, J, Puustinen, MC, Cheng, F, Sistonen, L, Nees, M. Oncogene 1–15 (2015).
siRNA mediated knockdown of HSF2 promotes prostate cancer 3D organoid invasion.
3. Latent KSHV Infected Endothelial Cells Are Glutamine Addicted and Require Glutaminolysis for Survival. Sanchez, EL, Carroll, PA, Thalhofer, AB, Lagunoff, M. PLOS Pathog. 11, e1005052 (2015).
A novel metabolic signature reveals a therapeutic target in Kaposi’s Sarcoma-associated Herpesvirus infected endothelial cells.
The number of IncuCyte™ references has surpassed 500! View the publications in each country and see which research institutions are using IncuCyte™ live-cell imaging.
We recommend using a CoolBox system (Cat. No. 4444) which provides a consistent temperature of 4-8°C for hours. CoolBox units are insulated boxes that hold a cold pack and either a microplate insert (CoolSink) or micro centrifuge rack (CoolRack), ensuring that the temperature across the invasion microplate is evenly maintained, thus reducing edge effects, and preventing matrix materials from polymerizing.
A: Visual edge effects can be eliminated by increasing the total media volume in each well (e.g. increasing well volume of a 96-well plate to 200 µL). The IncuCyte ZOOM® instrument will correctly quantify assay metrics regardless of volume. However, increasing the total media will improve image quality.
Our New Lab Startup Program is a program designed to help you get your new lab up and running. The program connects you with exclusive discounts and resources such as a discount on your first IncuCyte™ system order.
To verify your eligibility, please visit our website and register.
Our IncuCyte™ Referral Program is a program that rewards you when someone you refer to us, purchases an IncuCyte™ system.
For owners of IncuCyte™ IC and FLR instruments: Starting August 1, 2017, we will no longer offer extended warranties for IncuCyte™ IC and FLR instruments.
Instrument owners are eligible to purchase an upgrade for a limited time. Contact your local sales representative for more details or contact the IncuCyte™ service team.
Phone: +1 (734) 769-1600 - ext. 1
Phone: +44 1707 358688