Society for Neuroscience 2017
Booth #928
Visit our booth at SfN to learn the latest trends and applications in live-cell neuronal analysis using human iPSC-derived neurons, primary neurons, immortalized or neuronal-like cells in mono-cultures and co-cultures.
Change can happen in an instant. Find out how the IncuCyte® Live-Cell Analysis System and IncuCyte reagents enable you to visualize and quantify neuronal biology in real time. Derive deeper and more physiologically relevant information about neurite outgrowth, neural network stability and neuronal cell health - all inside your cell culture incubator!
At the conference, our R&D scientists will be presenting two posters showing innovative new research highlighting the powerful insights gained from IncuCyte® live-cell analysis. Learn how IncuCyte can help you confidently assess and measure neuronal dynamics, cell health and neuronal function without ever removing cells from the incubator!
Poster Presentations:
Long-term live-cell visualization and quantification of spontaneous synaptic activity and pharmacological response from human induced pluripotent stem cell-derived neuronal networks
Presenter: Aaron Overland, Ph.D., Senior Application Scientist
Session date and time: Wednesday, November 15, 2017 4:00 PM – 5:00 PM
Location: Halls A-C
Poster board number: VV81
Authors: Aaron C. Overland, John N. Rauch, Libuse Oupicka, Michael D. Uhler, David M. Rock, Daniel M. Appledorn
Abstract:
Recent advances in the differentiation and use of human induced pluripotent stem cell (hiPSC)-derived neurons have underscored their promise as an alternative in vitro model strategy for drug discovery and evaluating disease mechanisms of the human nervous system. However, iPSC-derived neurons are not well characterized, and the limitations of current instrumentation and biological protocols designed to measure their functional activity has revealed a clear need for more sophisticated methods that enable measurements of neuronal activity and connectivity over time with minimal perturbation. In this study, we used the IncuCyte S3® Live-Cell Analysis System to characterize spontaneous synaptic activity and connectivity from a variety of hiPSC-derived neuronal cell types and from rat E18 forebrain neurons in both monoculture and co-culture models.
Optimization of culture conditions and evaluation of cell health in human induced pluripotent stem cell-derived neurons using quantitative live-cell analysis
Presenter: John N. Rauch, Application Scientist
Session date and time: Tuesday, November 14, 2017 3:00 PM – 4:00 PM
Location: Halls A-C
Poster board number: W33
Authors: Aaron C. Overland, John N. Rauch, Libuse Oupicka, Michael D. Uhler, David M. Rock, Daniel M. Appledorn
Abstract:
The ability to culture, monitor and analyze cells that accurately reflect disease phenotypes of human nervous system disorders has been a major limitation in neuroscience research. The introduction of human induced pluripotent stem cell (hiPSC)-derived neurons has provided a promising new approach aimed at modeling human neurological diseases. However, hiPSC-derived neurons are not well characterized, and the capacity to monitor neuronal morphology and cell health is critical for the evaluation of these novel model systems in long-term culture. Traditional approaches rely on endpoint assays and imaging techniques that require immunochemical staining, yet they provide limited real-time kinetic information. In this study, we present data outlining optimal culture conditions for cell viability and neurite outgrowth in hiPSC-derived neurons from Cellular Dynamic International (CDI, iCell Neurons). We also evaluated neurite outgrowth and cellular viability in iCell Gluta Neurons from CDI using a quantitative, live-cell imaging approach with the IncuCyte S3® Live-Cell Analysis System over days/weeks in culture.