SFN 2018
Visit the Sartorius Booth #213 at SfN to learn the latest trends and applications in live-cell neuronal analysis, including our NEW Neuronal Activity Application.
Developing the next generation of therapies for brain disease relies on advanced technologies that provide detailed insights into the morphology, function and activity of living cells. The IncuCyte® S3 Live-Cell Analysis System for Neuroscience enables real-time automated measurements of the dynamic changes and interactions in living neural cells directly from the incubator.
Derive deeper insights into active biological processes with the IncuCyte S3 Live-Cell Analysis System for Neuroscience:
- Automated image acquisition and analysis of neuronal cell activity, cell health, structure and neuro-immune function in 96-and 384-well formats
- Visually verify morphological changes and validate measurements with images and movies
- Conduct week- or month-long studies on sensitive cells while they sit undisturbed in your incubator
At the show, our R&D scientists will be presenting a poster highlighting the power of live-cell analysis for quantification of dynamic neuro-immune function. Learn how IncuCyte® S3 Live-Cell Analysis System for Neuroscience and applications can enable new discoveries – all from inside your incubator!
Poster Presentation: Quantitative, live-cell kinetic analysis of microglial function and morphology
Presenter: Aaron Overland, Ph.D., Senior Application Scientist or John Rauch, Application Scientist
Session Date and Time: Sunday, November 4, 2018 10:00 AM – 11:00 AM
Location: SDCC Halls B-H
Poster Board Number: J2
Abstract:
Microglia, the resident immune cells of the central nervous system (CNS), play significant roles in the regulation of CNS homeostasis and in the management of tissue response to inflammatory or pathological insults. In the healthy unperturbed brain, microglia impact synaptic remodeling and turnover of dendritic spines through the removal of damaged or unnecessary neurons or synapses and are the first line of defense against pathogens through regulation of innate and adaptive immune responses. Upon insult, disease or stress, microglia can transform into an ‘activated’ state with altered phenotype and macrophage-like immune functions, including cytokine release and increased phagocytosis. Despite the importance of microglia in CNS regulation and disease, limited tools and in vitro model systems exist to enable optimizing, monitoring, and analyzing functional and morphological changes of these cells. In this study, we present data outlining optimization of plating conditions for in vitro microglial cultures, exemplified by the use of immortalized microglial cell lines (BV-2, HMC3 and C8-B4). We also evaluated the ability of BV-2, HMC3 and C8-B4 cells to phagocytose pHrodo-labeled E. coli bioparticles and apoptotic Neuro2A cells using a quantitative, live-cell imaging approach with the IncuCyte S3®. Activation of Toll-like receptor 4 (TLR-4) via the Gram-negative bacterial endotoxin lipopolysaccharide (LPS) resulted in variable microglial activation as measured by an altered phagocytic response. The ability of BV-2 cells to phagocytose apoptotic Neuro2A cells was diminished by pretreatment with an inhibitor of vitronectin receptors (Cilengitide) as well as an inhibitor of actin polymerization (cytochalasin D). Cilengitide treatment did not alter the ability of BV-2 cells to phagocytose E. coli bioparticles. Morphology, viability and proliferation were also monitored in real time over the course of the experiments. Proliferation of BV-2 cells was decreased with cytochalasin D treatment while remaining unaffected by Cilengitide. These results highlight the use of a quantitative, live-cell imaging approach with the IncuCyte S3® to monitor microglial function and morphology over the course of assay optimization and experimentation, thus allowing for improved developmental and pharmacological characterization.