Visit Sartorius at ECI 2018, booth #39, Amsterdam from 3rd to 5th September 2018 to learn the latest on the IncuCyte® S3 Live-Cell Analysis System and the Intellicyt® iQue Screener PLUS.
Our innovative cell analysis systems provide specialized instrumentation, software and reagents to accelerate discovery and development of novel therapeutics and provide new insight into the mechanisms of disease at a speed, depth and scale not achievable with conventional cell analysis techniques.
Derive deeper insights into active biological processes with the IncuCyte S3 Live-Cell Analysis System:
- Kinetic, image-based measurements from inside your incubator
- Real-time monitoring of cell health and viability, migration and invasion, and other phenotypic cell-based assays
- Profile cell-specific and time-dependent biological activity
- Powerful imaging & analysis tools for real-time decision-making
Also showcased is the Intellicyt iQue Screener PLUS.
Discover the fastest path to actionable results with our high throughput, suspension cell and bead analysis platform:
- Multiplexed binding assays (96-well plate in 5 min)
- Identify targets in their native conformation
- Evaluate specificity and species cross-reactivity in the same well
- Get information on cell health, isotype and titer from a single assay
At ECI, visit our poster presentation to learn about Quantifying immune cell subsets in living cultures over time using IncuCyte® live-cell analysis, presented by Clare Szybut.
Authors: C. Szybut, N. Bevan, H. Campwala, L. Kelsey, N. Dana, T. Jackson, N. Holtz, E. Endsley, T. Dale, D. Trezise. Essen BioScience, Welwyn Garden City, Hertfordshire UK & Ann Arbor, Michigan, USA
CD surface markers have long been used to identify immune cell subsets and associate cells with certain immune functions. Typically, flow cytometry and specific fluorescently-labelled anti-CD antibodies are used for these analyses. Whilst extremely powerful, this approach does not readily afford insight into temporal changes or spatial interactions between cell populations in heterogeneous systems. Here we describe a novel labelling and analysis strategy to enable long-term, non-invasive quantification of immune cells based on IncuCyte® live-cell imaging.
An Fc-targeted anti-mouse Fab fragment conjugated to a green-emitting fluoroprobe (IncuCyte FabFluor-488) was used to tag antibodies to cell surface markers. Addition of the FabFluor488-antibody complex to living cells, including OptiGreen background suppressor, produced fluorescent labelling that was sufficiently bright and stable to allow repeated measurements for >4 days without perturbing cell morphology or growth. With new image analysis and visualisation tools, individual cells were segmented from the phase-contrast image and quantified cell by cell for fluorescence. The method was validated by comparing the phase cell counts over time with nuclear fluorescence values in proliferating Jurkat-Nuclight RFP cells. In PBMCs, the anticipated frequencies of lymphocyte subpopulations (CD4, CD8, CD45) were detected using this method. Cell subsets can be gated for further analysis determining dynamic changes in response to stimuli, e.g. increase in CD71+ cells following anti-CD3/IL-2 activation.
FabFluor antibody labelling and live-cell imaging enables long-term tracking and analysis of immune cell subsets in living cultures over time. This method should prove powerful in analyses on dynamic heterogeneous cell models and for studying cell-cell interactions.