For the body to defend and fight against cancer, immune cells must recognize, engage, destroy and ultimately remove unwanted tumor cells. Understanding these processes at the cellular level is central to identifying and validating new immunotherapy approaches. Current methods for studying immune/tumor cell interactions are often end point, require cell lifting or measure indirect readouts meaning valuable data may be overlooked or compromised. This poster demonstrates how continuous live-cell analysis enables a new range of non-invasive phenotypic measurements for immuno-oncology research including: