Introducing the IncuCyte® Immune Cell Killing Assays

An integrated solution for real-time visualization and automated analysis of immune cell-mediated killing of tumor cells – all within your tissue culture incubator. Ideal for:

  • Cytotoxic T cell killing
  • Antibody-dependent cell-mediated cytotoxicity (ADCC)

T cell sub population of PBMCs

Labeling of the SK-OV-3 cancer cells with the NucLight Red Live-Cell Labeling Reagent

T cell killing of SK-OV-3 ovarian cancer cells. Two IncuCyte® movies showing a T cell sub population of PBMCs, activated with anti-CD3 antibody and IL-2, killing target SK-OV-3 tumor cells. (Left) The IncuCyte® Caspase 3/7 apoptosis reagent enables detection of cells undergoing apoptosis in real-time (green fluorescence). (Right) Labeling of the SK-OV-3 cancer cells with the NucLight Red Live-Cell Labeling Reagent enables simultaneous measurement of tumor cell proliferation.


Assay concept

  1. Target tumor cells are co-cultured with your immune cells of choice (e.g., T cells or human peripheral blood mononuclear cells; PBMCs).
  2. Tumor cell death is measured directly and in real-time by adding the mix-and-read IncuCyte® Caspase 3/7 Reagent to your cultures.
  3. Automated image analysis enables selective quantitation of tumor cell death. Optional: Simultaneous tumor cell counts can be enabled by labeling target cells with a range of NucLight™ Live-Cell Labeling Reagents.
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