During infection immune cells are guided toward sites of infection by a chemokine chemical trail. The IncuCyte live-cell analysis system enables automated, real-time analysis of immune cell chemotaxis and the movement of leukocytes across the endothelium at sites of inflammation (transendothelial migration). Ideal for the study of host-defense response and inflammatory disorders.
Cells are seeded onto the top surface of the IncuCyte™ ClearView membrane insert and monitored in real time using the IncuCyte® live cell analysis system as they migrate towards chemoattractant in the lower reservoir plate. Visualize and quantify cells on the top and bottom surface of the membrane.
Directed migration of primary T cells through a HUVEC endothelial cell monolayer toward the chemoattractant SDF-1α, using an IncuCyte® ClearView 96-well Cell Migration Plate. Cells were visualized and quantified in real time using the IncuCyte® Live Cell Analysis System. Automatically quantify the loss of leukocytes from the upper membrane surface as they move through the endothelial layer.