Immune Cell Migration Assays

During infection immune cells are guided toward sites of infection by a chemokine chemical trail. The IncuCyte live-cell analysis system enables automated, real-time analysis of immune cell chemotaxis and the movement of leukocytes across the endothelium at sites of inflammation (transendothelial migration). Ideal for the study of host-defense response and inflammatory disorders.

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IncuCyte® Chemotaxis Assays

• Monitor and quantify directional migration of immune cells across plastic or biomatrix coatings in real time
• The IncuCyte ClearView 96-well cell migration plate uses low numbers and supports mono- and co-cultures

Learn more about IncuCyte chemotaxis assays

Cells are seeded onto the top surface of the IncuCyte™ ClearView membrane insert and monitored in real time using the IncuCyte® live cell analysis system as they migrate towards chemoattractant in the lower reservoir plate. Visualize and quantify cells on the top and bottom surface of the membrane.

IncuCyte® Transendothelial Migration (TEM) Assays

• Visualize and automatically analyze directional leukocyte migration across an endothelial monolayer in real time
• Study host-defence response and inflammatory disorders in a 96-well format

Learn more about IncuCyte® transendothelial migration (TEM) assays

Directed migration of primary T cells through a HUVEC endothelial cell monolayer toward the chemoattractant SDF-1α, using an IncuCyte® ClearView 96-well Cell Migration Plate. Cells were visualized and quantified in real time using the IncuCyte® Live Cell Analysis System. Automatically quantify the loss of leukocytes from the upper membrane surface as they move through the endothelial layer.