Measurements of neurite outgrowth and cell viability are central to the study of drug candidates, culture conditions and the impact of environmental factors on neuronal cell health and function. Both neurite outgrowth and viability assays are routinely used in drug discovery screens and may be used to identify treatments that elicit neuroprotective effects but do not induce neurotoxicity.
IncuCyte® Neuronal Cell Health Assays enable automated, real-time multiplexed analysis of neurite dynamics and cell viability. Combine label-free readouts with non-perturbing live-cell reagents to simultaneously distinguish treatments that affect one or more aspects of neuronal cell health.
Measure neurite dynamics automatically and in real-time in neuronal monocultures and co-cultures with astrocytes.
Real time measurements of cell viability using simple mix-and-read protocols suitable for screening
Detect apoptosis in living cells and in real time using simple mix-and-read protocols
Measure changes in neurite length and branch points in mixed cultures of neurons and astrocytes using fluorescent neuronal labelling reagents and IncuCyte® analysis software.
Use IncuCyte® Cytotox and Annexin V reagents to positively mark and quantify apoptotic or cytotoxic cells. (Left) IncuCyte® Cytotox Red reagent labels dead rat forebrain neurons treated with 333 μM glutamate. (Right) IncuCyte® Annexin V Green reagent identifies apoptotic cells in a rat forebrain neuron and astrocyte co-culture in the presence and absence of 300 μM glutamate. Neurons are selectively labeled with the IncuCyte® NeuroLight™ Red reagent. Multiplexed measurements of cell viability and neurite dynamics can be made in real time.
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