Measure Chemotactic Migration, Invasion and Transendothelial Migration in Real Time
Use the IncuCyte® Live-Cell Analysis System to study chemotactic cell migration across 2D substrates, directional cell invasion through 3D biomatrix gels and directional leukocyte migration across the endothelium.
- Visualize chemotaxis in real time with fully automated analysis
- Use the ClearView chemotaxis plate for highly reproducible 96-well kinetic assays
- Low cell usage ideal for rare, expensive and primary cell populations
- Chemotactic gradients are stable and maintained over 72 hours
Chemotactic migration across substrate surfaces
- Monitor and quantify directional cell migration across plastic or biomatrix coatings in real time
- Validated with a wide range of immune, tumor and vascular cell types
- Study the effect of treatments on full time course chemotaxis profiles
Chemotactic invasion through 3D biomatrix gels
- Real time imaging and quantification of cell invasion through 3D biomatrix gels
- Assess metastatic potential and define the effect of treatments on invasive phenotype.
- Explore differential biology of chemotactic cell migration and invasion in the same plate.
Chemotatic transendothelial migration of leukocytes
- Monitor and quantify directional leukocyte migration across an endothelial monolayer in real time
- Visualize endothelial monolayer integrity and leukocyte diapedesis
- Investigate the biological effects of inhibitors and neutralizing antibodies
Commonly used approaches
In vitro assays for cell migration and chemotaxis are necessary for both basic-research and drug discovery. The table below shows capabilities and challenges of some common approaches.
| Existing Boyden Chambers | Microfluidic Devices | IncuCyte® Chemotaxis Cell Migration and Invasion | |
|---|---|---|---|
| Chemotactic migration |  |
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| Migration in the absence of chemotactic gradients | |
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| Low cell usage (<5,000 per well) | |
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| Suitable for non-adherent cells | |
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| Migration across a surface | |
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| Invasion through a 3D gel matrix | |
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| Integrated quantitation | |
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| Integrated cell observation | |
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| Precision, reproducibility | |
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| 96-well throughput or higher | |
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| Kinetic read-out | |
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| Label-free | |
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| Workflow: set up and walk away | |
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