TECH NOTE
Data processing analyzes and summarizes qualitative images into quantitative data. You will be required to define the analysis parameters (phase and/or fluorescence) for your assay plate. This task can be completed by using the Analysis Job Utilities found in the upper right-hand corner of the Vessel View window. The basic workflow of setting up an analysis job, from image collection to analysis launch, is represented below. Information icons are found throughout the software. Mouse over the information icons to find detailed information and useful tips.
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In the Vessel View window, review the scanned vessel and find representative images to add to an Image Collection.
Figure 1: Creating an Image Collection
A processing definition is a stored set of parameters that completely specifies how to process acquired images. Outlined below are the steps required to perform a phase-only analysis.
Figure 2: Chemaxis Migration (Top/Bot) Processing Definition Editor - PHASE
A processing definition is a stored set of parameters that completely specifies how to process acquired images. Outlined below are the steps required to perform a phase-only analysis
Figure 3: Chemotaxis Migration (Top Only) Processing Definition Editor - PHASE
A processing definition is a stored set of parameters that completely specifies how to process acquired images. Outlined below are the steps required to perform a fluorescence-only analysis.
Figure 4. Chemotaxis Migration (Top/Bot) Processing Definition Editor - FLUORESCENCE
A processing definition is a stored set of parameters that completely specifies how to process acquired images. Outlined below are the steps required to perform a fluorescence-only analysis.
Figure 5. Chemotaxis Migration (Top Only) Processing Definition Editor - FLUORESCENT
Once a processing definition has been defined for an assay, cell type, or magnification, Analysis Jobs can be performed.
Figure 6: Launching an Analysis Job (Vessel View).
After your scanned images have been analyzed, they can be used to generate graphs of the selected metric over time. The IncuCyte™ graphing feature can be accessed in the Vessel View window by either selecting the “Graph” tab and then clicking on the “Graph/ Export” button, or by choosing the Graph/Export option from the Utilities pull-down menu.
No assay metrics will be available until an analysis job is complete and opened. Open the Analysis Job at the bottom of the Vessel View window (notice that the color banner at the top of the Vessel View window changes from blue to green).
Metrics available for graphing are the same for both Chemotaxis
Migration (Top/Bot) and Chemotaxis Migration (Top Only) and are
outlined in detail below.
NOTE: When using the Chemotaxis Migration (Top/Bot) Analysis Job
Type, you have the ability to generate graphs for both the top-side
and bottom-side of the ClearView membrane.