PrimeKit - A cryopreserved 96-well human co-culture assay kit containing HUVECs + human dermal fibroblasts.
Follow tube formation in real time for up to 2 weeks via fluorescent quantification of GFP-labelled endothelial cells
Acquire and analyze images using fully integrated Angiogenesis software module. Visualize kinetic plots in microplate views. Export metrics (tube length, tube area, branch points) to calculate EC50/IC50 values
Study the inhibition of tube formation and/or investigate treatment effects on established networks. Quantify pro- and anti-angiogenic treatments
Concentration dependent vascular tube formation induced by Vasoactive Endothelial Growth Factor (VEGF) in PrimeKit HUVEC and human dermal fibroblast co-culture model. Upper left: Full time-course of VEGF induced vascular tube formation. Upper right: Microplate view to show 96-well assay layout and on plate consistency. VEGF was added at 60h. Abscissa: time (h), ordinate: tube length (mm/mm2). Lower left: Area under curve analysis of time-course data. Concentration-response curves (based on peak tube length) for VEGF and Avastin inhibition of VEGF (4 ng/ml) induced tube formation by Avastin (based on peak tube length). Lower right: Example image of VEGF (4 ng/ml) induced vascular structures.