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Stem Cell Monitoring & Reprogramming

Monitor stem cell reprogramming and differentiation events from your desktop without removing cells from the incubator. Identify colonies with IncuCyte® HD-phase contrast imaging and track colony formation, forward or backward in time.

3rd Edition! Live-Cell Analysis Handbook

A guide to real-time live-cell imaging & analysis

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Application Note:

Gain additional insights into the mechanism of immune cell killing by combining live cell analysis and flow cytometry into a single workflow

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Key features

Key features

Identify and track colonies

Inspect stem cells without removing them from the incubator. Identify colonies with high quality HD-phase imaging, all the way to the vessel edge

Capture reprogramming events

Monitor and calculate transduction and reprogramming efficiencies using fluorescent reporters and surface marker expression probes

Physically mark colonies

From your desk, use digital marking tool to locate and follow colonies over time

Remove laboratory bottlenecks

Manual inspection of wells using a stereomicroscope is laborious and time consuming. The IncuCyte® Live-Cell Analysis System allows for examination of wells via aquired images, and promotes collaboration with multiple user access

Example data

Example data

Upper left: Digitally highlight multiple areas of interest. Upper right: The IncuCyte system physically marks the vessel using the Plate Marking Tool. Lower left: Mark an emerging colony. Lower right: Digitally select the colony for physical marking.

Stem Cell Image - Petri dish + marked colony

IncuCyte HD-phase time-lapse images tracking colony evolution. Move back and forth in time to watch the history and evolution of emerging colonies.

Stem Cell Timelapse Image

Confirming pluripotency using cell surface markers. iPSCs derived from human adult dermal fibroblasts and grown on mouse embryonic fibroblast feeder cells. Double label confirmation of pluripotency. SSEA4 primary antibody paired + Alexa Fluor® 488 secondary antibody combined with OCT4 primary antibody + Alexa Fluor® 594 secondary antibody. Upper left: Image is the whole well overview. Upper right: Magnified image of two highlighted colonies from this well expressing both markers of pluripotency.

Stem Cell Monitoring & Reprogramming

Stem Cell Monitoring & Reprogramming

Technical Resources

Technical Resources

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