Use the IncuCyte® Live-Cell Analysis System to optimize and define cell culture regimes. Label-free phase segmentation metrics track cell growth over time using cell confluence curves
Collect HD-phase contrast images from microtiter plates, tissue culture flasks and dishes. Use a range of magnifications (4x, 10x, 20x objectives) to capture fine details of cells or spatial coverage of cell populations
Image multiple areas of your vessel or use whole-well imaging in microplates and dishes to highlight spatial variations and optimize cell distribution using quantitative metrics
Use IncuCyte to optimize and to minimize variations in cell seeding densities
Unveil time-dependent and transient events from your own desktop without the need to remove the vessels from your incubator
Acquire and analyze images using fully-integrated software algorithms. Define cell-type specific growth characteristics
Track cell proliferation over time using label-free phase segmentation (confluence) metric. Upper left: IncuCyte T-flask vessel view to show flask consistent cell growth. Upper right: Proliferation time-course for HT-1080 human fibrosarcoma cells. Lower panel: IncuCyte HD-phase time-lapse images of HT-1080 cells (confluence mask overlaid).
Growth curves for A172 human glioblastoma (upper left) and LNCap human prostate carcinoma (upper right) cells seeding at different densities. Lower panel: IncuCyte® HD-phase contrast images of A549 human lung carcinoma, HT-1080 human fibrosarcoma and LNCap cells shown without and with confluence mask overlaid in orange. Note the accurate confluence masking of all three cells types which have very different morphologies.