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Cell Culture QC

Label free, non-invasive approach to long-term monitoring of cell morphology and growth without removing cells from the incubator. Observe and understand time dependent and transient events.

4th Edition! Live-Cell Analysis Handbook

A guide to real-time live-cell imaging & analysis

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Application Note:

Gain additional insights into the mechanism of immune cell killing by combining live cell analysis and flow cytometry into a single workflow

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Key features

Key features

Optimize growth conditions

Use the IncuCyte® Live-Cell Analysis System to optimize and define cell culture regimes. Label-free phase segmentation metrics track cell growth over time using cell confluence curves

Amenable to multiple tissue culture vessels

Collect HD-phase contrast images from microtiter plates, tissue culture flasks and dishes. Use a range of magnifications (4x, 10x, 20x objectives) to capture fine details of cells or spatial coverage of cell populations

Accurate assessment of overall monolayer

Image multiple areas of your vessel or use whole-well imaging in microplates and dishes to highlight spatial variations and optimize cell distribution using quantitative metrics

Improve assay quality and consistency

Use IncuCyte to optimize and to minimize variations in cell seeding densities

Continuous monitoring

Unveil time-dependent and transient events from your own desktop without the need to remove the vessels from your incubator

Automated image acquisition & processing

Acquire and analyze images using fully-integrated software algorithms. Define cell-type specific growth characteristics

Example data

Example data

Track cell proliferation over time using label-free phase segmentation (confluence) metric. Upper left: IncuCyte T-flask vessel view to show flask consistent cell growth. Upper right: Proliferation time-course for HT-1080 human fibrosarcoma cells. Lower panel: IncuCyte HD-phase time-lapse images of HT-1080 cells (confluence mask overlaid).

Growth curves for A172 human glioblastoma and LNCap human prostate carcinoma cells seeding at different densities

Growth curves for A172 human glioblastoma (upper left) and LNCap human prostate carcinoma (upper right) cells seeding at different densities. Lower panel: IncuCyte® HD-phase contrast images of A549 human lung carcinoma, HT-1080 human fibrosarcoma and LNCap cells shown without and with confluence mask overlaid in orange. Note the accurate confluence masking of all three cells types which have very different morphologies.

Cell Culture QC

Cell Culture QC

Cell Culture QC - Multicell Phase Masked Images

Technical Resources

Technical Resources

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