This protocol provides an overview of the IncuCyte® Chemotaxis Cell Invasion Assay methodology and is compatible with the IncuCyte® ZOOM instrument. Measurements of chemotactic cell invasion can be made using nuclear labeled or unlabeled cells, however, we recommend labeling cells with a live-cell nuclear label (e.g. IncuCyte® NucLight™ reagents) to ensure optimal performance.
Air bubbles trapped on either side of the membrane can interfere with proper focusing and image processing. We recommend the following techniques to eliminate bubbles from your experiment: