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Kinetic Angiogenesis Movies captured on Essen IncuCyte™

 

Control Well- CellPlayer™ Angiogenesis Assay:
GFP-AngioKit co-culture assay with NHDF fibroblasts and GFP-labeled HUVECs stimulated with 4 ng/mL VEGF and imaged every 12 hours for 17 days. This movie demonstrates the kinetic process of tube formation as measured by IncuCyte over the course of the assay.

 

Suramin Treated Well- CellPlayer™ Angiogenesis Assay:
GFP-AngioKit co-culture assay with NHDF fibroblasts and GFP-labeled HUVECs treated with 20 micromolar Suramin in the presence of 4 ng/mL VEGF and imaged every 12 hours for 17 days. Suramin, a prototypic inhibitor of growth factor signaling, significantly decreases VEGF potentiation of tube formation

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