Control Well- CellPlayer™ Angiogenesis Assay:
GFP-AngioKit co-culture assay with NHDF fibroblasts and GFP-labeled HUVECs stimulated with 4 ng/mL VEGF and imaged every 12 hours for 17 days. This movie demonstrates the kinetic process of tube formation as measured by IncuCyte over the course of the assay. |
Suramin Treated Well- CellPlayer™ Angiogenesis Assay:
GFP-AngioKit co-culture assay with NHDF fibroblasts and GFP-labeled HUVECs treated with 20 micromolar Suramin in the presence of 4 ng/mL VEGF and imaged every 12 hours for 17 days. Suramin, a prototypic inhibitor of growth factor signaling, significantly decreases VEGF potentiation of tube formation |